Composition and methods for imaging cells
AssigneesCase Western Reserve University, Northwestern University
InventorsJames Basilion, Kirti Dhingra, Thomas J. Meade, Sarah Grace Kamper
Life of this patent
Estimating the term…
Abstract
A composition for imaging a cell includes a first imaging probe and a second imaging probe that include respectively a first reporter moiety and a second reporter moiety. The first reporter moiety and the second reporter moiety form a signaling complex that produces a detectable signal when the first imaging probe and second imaging probe complex with first and second biomarkers of the cell.
Claims22
1-32. (canceled)
A method for imaging a cell having a first biomarker and a second biomarker that is different than the first biomarker, the method comprising the steps of:
- contacting the cell with an imaging composition comprising first and second imaging probes, the first imaging probe comprising a first targeting moiety that is linked to an enzyme-activated, contrast agent via a linker region, the first targeting moiety specifically complexing with the first biomarker, the second imaging probe comprising a second targeting moiety that is linked to an activator molecule via a linker region, the second targeting moiety being different from the first targeting moiety and specifically complexing with the second biomarker, the enzyme-activated contrast agent and the activator molecule forming a signaling complex that produces a detectable signal when the first imaging probe and second imaging probe complex with first and second biomarkers of the cell;
- and
- detecting a signal, the signal being generated when the first imaging probe and second imaging probe complex with first and second biomarkers of the cell.
A method for determining a molecular signature of a cell that is associated with disease, the cell having a first biomarker and a second biomarker that is different than the first biomarker, the method comprising the steps of:
- contacting the cell with an imaging composition, the imaging composition comprising first and second imaging probes, the first imaging probe comprising a first targeting moiety that is linked to an enzyme-activated, self-immolative contrast agent via a linker region, the first targeting moiety specifically complexing with the first biomarker, the second imaging probe comprising a second targeting moiety that is linked to an activator molecule via a linker region, the second targeting moiety being different than the first targeting moiety and specifically complexing with the second biomarker, the enzyme-activated, self-immolative contrast agent and the activator molecule forming a signaling complex that produces a detectable signal when the first imaging probe and second imaging probe complex with first and second biomarkers of the cell;
- detecting a signal, the signal being generated when the first imaging probe and second imaging probe complex with first and second biomarkers of the cell;
- and
- producing the molecular signature of the cell based on the detectable signal, the molecular signature indicating at least one of the type, stage, or severity of the disease.
44-56. (canceled)
Dependencies are inferred from the claim text.