IPSC-Based Gamma-Delta T-Cells, Compositions and Methods of Use Thereof
AssigneeIN8bio, Inc.
InventorsYanjie LI, Lei DING, Lawrence S. LAMB, Jr.
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Abstract
Described are methods of preparing precursor cell-derived induced pluripotent stem cells (iPSCs) and methods of generating iPSC-derived gd T-cells. The invention also includes cell populations comprising the iPSCs, cell populations comprising the iPSC-derived gd T-cells, pharmaceutical compositions comprising the cell populations, and methods of treating cancer comprising administration of the cell population or pharmaceutical composition thereof.
Claims28
A method of generating functional iPSC-derived γδ T-cells comprising the steps of:
- a. preparing a population of precursor cell-derived induced pluripotent stem cells (iPSCs), the method comprising the steps of:
- i. isolating a population of precursor cells and culturing the precursor cells in a culturing medium, wherein the precursor cells are human somatic cells;
- ii. transducing the precursor cells with a non-integrating virus vector encoding a plurality of reprogramming factors, optionally comprising one or more of Oct3/4, Sox2, Kfl4 and c-Myc;
- and
- iii. culturing the transduced precursor cells under conditions suitable for reprogramming the cells to pluripotency to obtain the population of precursor cell derived-iPSCs;
- and
- b. differentiating the precursor cell-derived iPSCs to CD34+ hematopoietic progenitor cells (HPCs) and generating progenitor T-cells and functional iPSC-derived γδ T-cells therefrom under feeder-free conditions, wherein the iPSC-derived γδ T-cells comprise γδ1 T-cells and/or γδ2 T-cells.
A method of preparing a population of precursor cell-derived induced pluripotent stem cells (iPSCs), comprising:
- i. isolating a population of precursor cells and culturing the precursor cells in a culturing medium, wherein the precursor cells are human somatic cells;
- ii. transducing the precursor cells with a non-integrating virus vector encoding a plurality of reprogramming factors, optionally comprising one or more of Oct3/4, Sox2, Kfl4 and c-Myc;
- and
- iii. culturing the transduced precursor cells under conditions suitable for reprogramming the cells to pluripotency to obtain the population of precursor cell derived-iPSCs.
Dependencies are inferred from the claim text.