Expression of eimeria sequences in plants and plant produced vaccine for same
AssigneeMazen Animal Health Inc.
InventorsJohn Howard, Erin Egelkrout, Joan Schrader
Life of this patent
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Abstract
Vaccines and methods of expressing a polypeptide of Eimeria are provided in which a protective response to Eimeria is produced when administered to an animal. The vaccine provides for expression of Eimeria vaccine proteins 3-1e, Gam82, and/or EF-1a polypeptide in a plant or plant part, linked to a promoter preferentially directing expression to embryo tissue of the plant or plant part. Further embodiments provide that the polypeptide may be targeted to the apoplast/cell wall or the endoplasmic reticulum. Increased expression levels in the plant or plant part are obtained. The plant or plant materials in an embodiment may be orally administered.
Claims21
A method of producing a protective response to Eimeria in an animal, the method comprising:
- a) orally administering to said animal a composition comprising plant or plant product comprising at least one Eimeria vaccine protein, or a sequence having at least 90% identity thereto or a functional fragment of said at least one Eimeria vaccine protein expressed at levels of at least 10 mg/kg in seed of said plant;
- and
- b) producing a protective response to said Eimeria in said animal.
A vaccine comprising a plant-produced polypeptide of Eimeria vaccine protein, the vaccine comprising a plant or plant product comprising a construct comprising,
- (a) a promoter preferentially directing expression to embryo tissue of a plant;
- (b) a nucleic acid molecule encoding Eimeria vaccine protein as disclosed herein, or a sequence having at least 90% identity thereto or a functional fragment operably linked to said promoter;
- and
- (c) a nucleic acid molecule targeting expression of said polypeptide in the apoplast or endoplasmic reticulum of said plant;
- and expressing said Eimeria vaccine protein in said plant at levels of at least 1 mg/kg of seed of said plant.
A method of expressing a polypeptide of Eimeria vaccine protein or a functional fragment thereof, the method comprising introducing into a plant a construct comprising,
- (a) a promoter preferentially directing expression to embryo tissue of a plant;
- (b) a nucleic acid molecule encoding an Eimeria vaccine protein as disclosed herein or a sequence having at least 90% identity thereto or a functional fragment operably linked to said promoter;
- and
- (c) a nucleic acid molecule targeting expression of said polypeptide in the apoplast and/or endoplasmic reticulum of said plant;
- and expressing said Eimeria vaccine protein in said plant at levels of at least 1 mg/kg of seed of said plant.
Dependencies are inferred from the claim text.