Methods of embryo multiplication
AssigneeNbryo Pty Ltd
InventorsWilhelmus TE BRAKE, Nicholas CAMERON, Danielle AZOULAY, Margot DAY, Michael MORRIS
Life of this patent
Estimating the term…
Abstract
The present disclosure relates generally to methods of producing multiple embryos from one or more donor embryos comprising embryonic cells that are developmentally equivalent to embryonic cells from a 16-cell embryo or a pre-compacted morula. More particularly, the method of the disclosure comprises unzipping said donor embryos and expanding one or more aggregates of blastomeres obtained from the donor embryo(s) to produce multiple blastocysts from the donor embryo(s). The present disclosure also relates to the use of such methods in animal breeding.
Claims33
A method of multiplying a donor embryo, said method comprising:
- (i) obtaining a donor embryo comprising one or more embryonic cells that are developmentally equivalent to embryonic cells from a 16-cell embryo or a pre-compacted morula;
- (ii) separating a plurality of the embryonic cells from the donor embryo;
- (iii) expanding the embryonic cells in vitro under conditions suitable to produce a plurality of conceptuses from the donor embryo, wherein at least a portion of the embryonic cells are expanded as one or more cell aggregates, each aggregate comprising 2 or more embryonic cells;
- and
- (iv) culturing the plurality of conceptuses under conditions suitable to produce a plurality of blastocysts.
Dependencies are inferred from the claim text.