Method for enhancing a raman contribution in a spectrum, spectroscopy system, computer program and non-transitory computer-readable storage medium

A spectroscopy system comprising:

• a light source;
• a focusing element configured to direct light emitted by the light source towards a sample, wherein a longitudinal position of a focal point of the focusing element is adjustable;
• a spectrometer configured to record a spectrum of light;
• and
• circuitry configured to execute:
• a first focal point function configured to set a first longitudinal position of the focal point of the focusing element, whereby a first spot size of light emitted by the light source at a first position of the sample is set,
• a first record function configured to record, using the spectrometer, a first spectrum of light comprising information associated with ambient light and a first measurement response, wherein the first measurement response comprises a first optical response and a first Raman response of the sample in response to being illuminated with light having the first spot size,
• a second focal point function configured to set a second longitudinal position of the focal point of the focusing element, whereby a second spot size of light emitted by the light source at a second position of the sample is set,
• a second record function configured to record, using the spectrometer, a second spectrum of light comprising information associated with ambient light and a second measurement response, wherein the second measurement response comprises a second optical response and a second Raman response of the sample in response to being illuminated with light having the second spot size;
• and
• wherein the first spot size is larger than the second spot size, whereby an intensity of light from the light source at the sample is stronger within the second spot size than within the first spot size, whereby a contribution, to the first measurement response, of the first Raman response in relation to a contribution of the first optical response is smaller than a contribution, to the second measurement response, of the second Raman response in relation to the second optical response,
• wherein the circuitry is further configured to execute a form function configured to form a data set comprising information associated with a spectrum of the sample based on a dissimilarity between the first spectrum and the second spectrum, whereby a contribution of a Raman response to the formed data set is enhanced.

A computer program stored on a computer-readable storage medium, the computer program comprising program code portions configured to cause circuitry of a Raman spectroscopy system to execute a method, comprising:

• setting a first spot size of light emitted by a light source at a first position of the sample;
• recording a first spectrum of light comprising information associated with ambient light and a first measurement response, wherein the first measurement response comprises a first optical response and a first Raman response of the sample in response to being illuminated with light having the first spot size;
• setting a second spot size of light emitted by the light source at a second position of the sample;
• recording a second spectrum of light comprising information associated with ambient light and a second measurement response, wherein the second measurement response comprises a second optical response and a second Raman response of the sample in response to being illuminated with light having the second spot size;
• wherein the first spot size is larger than the second spot size, whereby an intensity of light from the light source at the sample is stronger within the second spot size than within the first spot size, whereby a contribution, to the first measurement response, of the first Raman response in relation to a contribution of the first optical response is smaller than a contribution, to the second measurement response, of the second Raman response in relation to the second optical response;
• and
• forming a data set based on a dissimilarity between the first spectrum and the second spectrum, thereby enhancing a contribution of a Raman response to the formed data set.