Polypeptides for use in the synthesis of kratom alkaloids
AssigneeMitradyne Corporation
InventorsJose CASARETTO, Tariq A. AKTAR, Steven ROTHSTEIN, Eric SOUBEYRAND
Life of this patent
Estimating the term…
Abstract
Described herein are polypeptides encoding enzymes for the synthesis of monoterpene indole alkaloids. For example, the polypeptide comprises or consists of the sequence of SEQ ID NO: 1-68, or a variant thereof having at least 80% sequence identity to SEQ ID NO: 1-68, or a fragment of the polypeptide, or the variant thereof.
Claims20
A polypeptide encoding a strictosidine glucosidase that removes the glucose moiety from strictosidine to produce strictosidine aglycone.
A polypeptide encoding a geissoschizine synthase that opens the tetrahydropyran ring of strictosidine aglycone to produce geissoschizine.
A polypeptide encoding a hydroxylase that hydroxylates the indole ring of corynantheidine at the C9 position to produce 9-hydroxycorynantheidine.
A polypeptide encoding a hydroxylase that hydroxylates the indole ring of mitragynine at the C7 position to produce 7-hydroxymitragynine.
A polypeptide encoding an O-methyltransferase that methylates the hydroxyl group at the C9 position of 9-hydroxycorynantheidine to produce mitragynine.
Dependencies are inferred from the claim text.