Perfusion Bioprocessing System and Method of Operating the Same
AssigneeCytiva Sweden AB
InventorsPraveen Paul, Prashanth Hosabettu Mohan, Ajay Gore, Sahebagouda Alagur
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Abstract
A perfusion bioprocessing system (10) includes a bioreactor (12) and a recirculation flow path (14) provided with at least one first feed flow control device (46) and at least one second feed control device (48). The perfusion bioprocessing system (10) further includes a first tangential flow filter (16) coupled to the bioreactor (12) via the recirculation flow path (14) and a second tangential flow filter (18) coupled to the bioreactor (12) via the recirculation flow path (14). The first tangential flow filter and the second tangential flow filter are coupled to a permeate flow path and a retentate flow path.
Claims20
A method for automatic operation of a perfusion bioprocessing system, the method comprising steps of:
- inducing a first quantity of a process fluid for a cell culture to flow from a bioreactor to a first tangential flow filter via a recirculation flow path;
- operating a control unit to control at least one first feed flow control device in the recirculation flow path for controlling the flow of the first quantity of the process fluid;
- allowing the first tangential flow filter to separate the first quantity of the process fluid into a first quantity of a permeate fluid and a first quantity of a retentate fluid;
- inducing a second quantity of the process fluid to flow from the bioreactor to a second tangential flow filter via the recirculation flow path;
- operating the control unit to control at least one of the at least one first feed flow control device and at least one second feed control device in the recirculation flow path for controlling the flow of the second quantity of the process fluid;
- and
- allowing the second tangential flow filter to separate the second quantity of the process fluid into a second quantity of the permeate fluid and a second quantity of the retentate fluid.
Dependencies are inferred from the claim text.