Edited nac genes in plants
AssigneePioneer Hi-Bred International, Inc.
InventorsZHENGLIN HOU, MARY A RUPE, BO SHEN, ROBERT W WILLIAMS, WEIQING ZENG, JUN ZHANG
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Abstract
Compositions and methods for editing an endogenous NAC genes in plants are provided, for the improvement of traits of agronomic or commercial importance. Modifications of expression and activity of NAC genes are described, including editing endogenous NAC gene functional motifs and knocking out NAC gene function.
Claims21
-20. (canceled)
A method of modifying an endogenous genomic locus of a maize plant, wherein the locus comprises a polynucleotide encoding a NAC7 polypeptide, said method comprising:
- a) providing to at least one cell of the maize plant a Cas endonuclease, and
- b) introducing one or more genetic modifications at the genomic locus that results in reduced activity of the NAC7 polypeptide, as compared to a control maize plant not comprising the one or more genetic modifications,
- wherein the NAC7 polypeptide comprises a sequence that shares at least 95% sequence identity with SEQ ID NO:3.
A method of altering the binding specificity of a NAC7 protein in a maize plant, the method comprising introducing one or more edits to a central β-sheet β4 edge motif comprising N1-N2-N3-N4-N5-N6-N7-N8-N9, wherein:
- a) N1=F, R, T, V, or Y;
- b) N2=W;
- c) N3=H, K, R, N, or S;
- d) N4=S, P, T, I, A, or K;
- e) N5=T, S, A, V, or E;
- f) N6=G, A, or C;
- g) N7=R, K, A, S, T, P, or N;
- h) N8=D, S, T, E, or P;
- or
- i) N9=K, E, C, G, T, or R.
Dependencies are inferred from the claim text.