Phenolic compounds substituted with non-radioactive isotopes and uses thereof

Abstract

The present invention relates to phenolic compounds substituted with non-radioactive isotopes and the uses thereof, and more specifically to phenolic compounds in which some elements of biotin-phenol or desthiobiotin-phenol are substituted with non-radioactive isotopes and the uses thereof as probes for APEX family enzymes used in proximity molecular labeling. According to the present invention, proteins that are present in spaces that are not separated by membranes (e.g., mitochondrial cristae lumen), which previously could not be analyzed, can be identified, and the quantitative comparative analysis of protein expression in cells in different environments is possible, and it has the advantage of being able to quantitatively compare and analyze protein ratios in different adjacent spaces, and particularly, it has the advantage of being able to label proteins economically compared to the conventional technique of labeling proteins using heavy-carbon labeled amino acids.

Dependencies are inferred from the claim text.