Systems and methods for generation of hyperpolarized materials

A method for preparing a hyperpolarized biorelevant imaging agent or a pharmaceutically acceptable salt thereof, the method comprising:

• (a) providing a composition comprising a compound of Formula (II):
• wherein Z′ is:
• (i) a parahydrogenated carbon-carbon single bond (—CH*—CH*—) which is substituted to include 1H (proton), 2H (deuterium), or a combination thereof, or (ii) a parahydrogenated carbon-carbon double bond (—CH*═CH*—) which is substituted to include 1H (proton), 2H (deuterium), or a combination thereof;
• wherein H* is a hydrogen having a spin order derived from parahydrogen:
• R1 comprises a parahydrogen induced polarization (PHIP) transfer moiety;
• R2 comprises an optionally substituted hydrocarbon, alkoxy group, primary amine, secondary amine, or tertiary amine;
• and
• R3 comprises a biorelevant imaging agent comprising a non-hydrogen nuclear spin;
• and
• (b) applying a polarization transferring waveform to transfer nuclear spin order from at least one H* in the compound of Formula II to the non-hydrogen nuclear spin,
• thereby forming a derivative of Formula II having a hyperpolarized biorelevant imaging agent.