Chromatography medium for purifying target biologics
AssigneeNorth Carolina State University
InventorsStefano Menegatti, Jacob P. LeBarre
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Abstract
The present disclosure provides materials and methods related to the purification of a biologic. The present disclosure provides materials and methods related to the purification of a biologic. In particular, the present disclosure provides a multifunctional chromatography medium comprising porous beads or particles having a defined pore diameter distribution and one or more ligands conjugated to the surface of the pores capable of removing process-related impurities and product-related impurities from a target biologic.
Claims35
A chromatography medium comprising:
- a solid substrate having pores, wherein the solid substrate has a defined pore diameter distribution;
- and
- one or more ligands conjugated to a surface of the pores.
A method of selecting a multifunctional chromatography medium for purifying a target biologic comprising:
- determining a molecular weight or size of one or more impurities in a sample comprising the target biologic;
- identifying at least one additional characteristic of the one or more impurities;
- selecting the multifunctional chromatography medium based on the molecular weight or size and at least one additional characteristic of the impurities which leads to separation of the target biologic from the one or more impurities in a single step.
Dependencies are inferred from the claim text.