Method of purification and purified products

A method of packing a chromatography column in column chromatography comprising:

• adding a stationary phase to a column, and
• allowing a slurry comprising the stationary phase to settle in the column for a standing time, wherein the stationary phase is a reverse phase silica stationary phase, and the compound to be purified by column chromatography is selected from fatty acids derived from fish oil or esterified derivatives thereof, proteins and nucleic acids, and wherein chromatography comprises elution with a mobile phase comprising one or more of water, methanol, ethanol, or acetonitrile.

A method of increasing theoretical plate number (N) in a column chromatography comprising:

• adding a stationary phase to a column, and
• allowing a slurry comprising the stationary phase to settle in the column for a standing time, wherein the increase in N is relative to a column chromatography without standing time or wherein the increase in N is relative to a slurry that settles for a reduced standing time that is less than the standing time, wherein the stationary phase is a reverse phase silica stationary phase, and the compound to be purified by column chromatography is selected from fatty acids derived from fish oil or esterified derivatives thereof, proteins and nucleic acids, and wherein chromatography comprises elution with a mobile phase comprising one or more of water, methanol, ethanol, or acetonitrile.

A method of improving symmetry factor of a chromatographic peak in column chromatography comprising allowing a slurry to settle in the column for a standing time,

• wherein the improvement comprises decreasing the symmetry factor of the peak to a quantity >1, and wherein the decrease is relative to a symmetry factor when a column chromatography comprises a reduced standing time that is absent or less than the standing time and the symmetry factor is >1;
• or
• the improvement comprises increasing the symmetry factor of the peak to a quantity ≤1, and wherein the increase is relative to a symmetry factor when a the column chromatography comprises a reduced standing time that is absent or less than the standing time and the symmetry factor is <1, and wherein the slurry comprises a stationary phase that is a reverse phase silica stationary phase, and the compound to be purified by column chromatography is selected from fatty acids derived from fish oil or esterified derivatives thereof, proteins and nucleic acids, and wherein chromatography comprises elution with a mobile phase comprising one or more of water, methanol, ethanol, or acetonitrile.