Single process for sequential extraction of products from green tea leaves
OwnerMohit Sharma, Mehak Sharma +1inventors
InventorsMohit Sharma, Mehak Sharma, Vivesh Sood
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Abstract
The present invention discloses an integrated, cost effective process for simultaneous isolation and purification of wax, chlorophyll, Catechin from fresh green tea (Camellia sinensis) leaves. Further, process for isolation and purification of caffeine from the residual tea leaves left after the extraction of above said products. Optimization of various process parameters has also been done. In addition to this the quality of products was also evaluated on the basis of TLC, FTIR, HPLC, GC-MS, method.
Claims10
1-11. (canceled)
An integrated process for sequentially extracting products from green tea leaves, the integrated process comprising:
- (a) providing green tea leaves obtained from Camellia sinensis;
- (b) dipping the green tea leaves in an alkane solvent for 2 hours to 3 hours at room temperature to obtain a solution;
- (c) filtering the solution of (b) to obtain a filtrate and residual leaves;
- (d) drying the filtrate of (c) to obtain a wax as a first product;
- (e) dipping the residual leaves of (c) in alcohol followed by heating at 30° C. to 42° C. for 1 hour to 2 hours to obtain a green-colored solution and leftover leaves;
- (f) drying the green-colored solution of (e) to obtain a coloring pigment as a second product;
- (g) drying the leftover leaves of (e) followed by hydroextraction in a ratio 1:10 at 60° C. for 30 minutes to obtain a first extract and residual spent leaves;
- (h) purifying the first extract of (g) by micro filtration with a 0.1-micron to 0.5-micron pore size to obtain a purified first extract;
- (i) passing the purified first extract of (h) through a polystyrene adsorbent Resin-A or through a Resin-C;
- (j) eluting the purified first extract of (h) with alcohol to obtain a brown-colored catechin powder as a third product;
- (k) hydroextracting the residual spent leaves of (g) with sodium carbonate at 100° C. for 1 hour to obtain a second extract;
- and
- (l) cooling the second extract of (k) and recovering caffeine as a fourth product by liquid-liquid extraction of the second extract with dichloromethane solvent.
Dependencies are inferred from the claim text.