Methods for production of platelets from pluripotent stem cells and compositions thereof
AssigneeAstellas Institute for Regenerative Medicine
InventorsQiang Feng, Shi-Jiang Lu, Robert P. Lanza
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Abstract
Methods for production of platelets from pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) are provided. These methods may be performed without forming embryoid bodies or clusters of pluripotent stem cells, and may be performed without the use of stromal inducer cells. Additionally, the yield and/or purity can be greater than has been reported for prior methods of producing platelets from pluripotent stem cells. Also provided are compositions and pharmaceutical preparations comprising platelets, preferably produced from pluripotent stem cells.
Claims21
-73. (canceled)
A composition comprising a plurality of cells, wherein at least 70% of the cells in the composition are CD41a(+), CD42b(+) megakaryocytes having a knockout of endogenous β2 microglobulin, wherein the CD41a(+), CD42b(+) megakaryocytes are human megakaryocytes.
Dependencies are inferred from the claim text.