Wearable biosensor device and method for detection and measurment of bio-molecules and bio-particles

A system for sweat collection and quantification of sweat composition, comprising:

• a wearable fluid device configured for direct contact with a skin surface, and configured to collect of sweat from multiple skin points or store the collected sweat in small volumes and with preserved molecular contents;
• and
• a biosensor comprising a plurality of electrodes in communication with the wearable sweat-collecting fluid device, configured to sense one more target biomolecules and/or bioparticles in the collected sweat;
• and
• a potentiostat readout device in communication with the biosensor and comprising a transceiver for communicating data associated with an output the biosensor electrodes.

A system for rapid and controllable sweat collection along with a label-free and accurate quantification of sweat composition, comprising:

• a disposable, self-powered, and flexible wearable fluid device that is easily mountable and reliably sealable on a skin surface, enabling the collection of the sweat from multiple skin points and/or storing it within 1 nL-100 uL volume with preserved molecular contents with or without need for physical activities;
• an electrochemical biosensor with miniaturized, flexible, and multiplexed screen-printed electrodes, compatible with the flexible sweat-collecting fluid device, enabling continuous, longitudinal and/or single-point sensing of one or multiple target biomolecules and/or bioparticles in the collected sweat;
• and
• a miniaturized potentiostat readout device that is mounted inside a watch or a fitness band, and is easily assembled on the biosensor with communication capability.