# US 2025/0085294 A1 · Protein scaffold capable of carrying radioactive signals and application in antibody detection

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- Number: US 2025/0085294 A1
- Document ID: US20250085294A1
- Status: Published application
- Kind: A1
- Application number: 18/829,320
- Filed: 2024-09-10
- Published: 2025-03-13
- Earliest claimed: 2023-09-11
- Assignees: Jiangsu Province Hospital the First Affiliated Hospital With Nanjing Medical University
- Applicants: Jiangsu Province Hospital the First Affiliated Hospital With Nanjing Medical University
- Inventors: Heng CHEN; Tao YANG; Yong GU
- Web page: https://patentgrep.com/patent/US20250085294A1

## CPC

- G01N 33/6854: {Immunoglobulins}
- C07K 14/001: {by chemical synthesis}
- G01N 33/60: involving radioactive labelled substances
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

A protein scaffold capable of carrying radioactive signals and application thereof in antibody detection are provided. The present disclosure successfully finds out a protein scaffold R12 capable of carrying radioactive signals, which is used for assisting part of antigens to capture corresponding antibodies; and because the method is used for carrying signals and eliminating more severe screening conditions of cross reaction in the screening process, the method is not limited to the method in application, and the method can be directly used for a technical platform which only needs to eliminate the cross reaction.

## Claims

### Claim 1

(canceled)

### Claim 2

(canceled)

### Claim 3

An application of a protein scaffold in a detection kit for an anti-butyrophilin 3A1 antibody, wherein the detection kit for the anti-butyrophilin 3A1 antibody comprises a radioactive antigen protein AR12A, the radioactive antigen protein AR12A is obtained by labelling the antigen protein AR12A with S35-methionine, and a gene sequence of the antigen protein AR12A comprises a gene sequence of the protein scaffold and a gene sequence of the BTN3A1;
the amino acid sequence of the protein scaffold is as follows:
IKNILQPGSVDSQTEMVLVNAVYFKGMWEKAFKDEDTQAMPFRMT
EQESTPVQMMYQVGSFKVAEMASEKMKILELPYASGELSMLVLLP
DDVSGLEEIENAITFEKLTEWTSSSIMEERKIKVYLPRMKMEEKY
NLTSVLMALGMTDLF;
the amino acid sequence of the antigen protein AR12A is as follows:
LLGGAGYFLWQQQEEKKTQFRKKKREQELREMAWSTMKQEQSTRV
KLLEELRWRSIQYASRGERHSAYNEWKKALFKPADVILDPKTANP
ILLVSEDQRSVQRAKEPQDLPDNPERFNWHYCVLGCESFISGRHY
WEVEVGDRKEWHIGVCSKNVQRKGWVKMTPENGFWTMGLTDGNKY
RTLTEPRTNLKLPKPPKKVGVFLDYETGDISFYNAVDGSHIHTFL
DVSFSEALYPVFRILTLEPTALTICPAPKPSTPPGSSGGGSIKNI
LQPGSVDSQTEMVLVNAVYFKGMWEKAFKDEDTQAMPFRMTEQES
TPVQMMYQVGSFKVAEMASEKMKILELPYASGELSMLVLLPDDVS
GLEEIENAITFEKLTEWTSSSIMEERKIKVYLPRMKMEEKYNLTS
VLMALGMTDLFPKPSTPPGSSGGGSLLGGAGYFLWQQQEEKKTQF
RKKKREQELREMAWSTMKQEQSTRVKLLEELRWRSIQYASRGERH
SAYNEWKKALFKPADVILDPKTANPILLVSEDQRSVQRAKEPQDL
PDNPERFNWHYCVLGCESFISGRHYWEVEVGDRKEWHIGVCSKNV
QRKGWVKMTPENGFWTMGLTDGNKYRTLTEPRTNLKLPKPPKKVG
VELDYETGDISFYNAVDGSHIHTFLDVSFSEALYPVFRILTLEPT
ALTICPA.

### Claim 4 (depends on 3)

The application according to claim 3, wherein the detection kit further comprises a TBST buffer solution, a protein A agarose, a protein G agarose, a positive control and a negative control.