# US 2025/0085271 A1 · Optical multianalyte detection > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2025/0085271 A1 - Document ID: US20250085271A1 - Status: Published application - Kind: A1 - Application number: 18/464,185 - Filed: 2023-09-08 - Published: 2025-03-13 - Earliest claimed: 2023-09-08 - Assignees: Truvian Sciences, Inc. - Applicants: Truvian Sciences, Inc. - Inventors: Dena Marrinucci; Nicholas Haase; Ryan Morgan; Michael Adams; Derek Arndt; Graeme Cross; Greg Grabarek; Jeffrey A. Hawkins; David Hecht; Luke Marsden; Robert Neaves; Johnny Steve Rachele; Jay Srinivasan; Roger Taylor; Scott Thompson - Web page: https://patentgrep.com/patent/US20250085271A1 ## CPC - B01L 3/5025: {for parallel transport of multiple samples} - G01N 33/492: {Determining multiple analytes} - B01L 3/502: {with fluid transport, e.g. in multi-compartment structures} - B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces} - B01L 3/527: {for a plurality of reagents} - C12Q 1/6816: characterised by the detection means (C12Q1/6804 takes precedence) - G01N 21/6428: {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53)} - B01L 2200/04: Exchange or ejection of cartridges, containers or reservoirs - B01L 2200/0642: Filling fluids into wells by specific techniques - B01L 2200/143: Quality control, feedback systems - B01L 2200/16: Reagents, handling or storing thereof - B01L 2300/021: Identification, e.g. bar codes - B01L 2300/0627: Sensor or part of a sensor is integrated - B01L 2300/0645: Electrodes - B01L 2300/0663: Whole sensors - B01L 2300/0829: Multi-well plates; Microtitration plates - B01L 2300/0864: comprising only one inlet and multiple receiving wells, e.g. for separation, splitting - B01L 2300/1827: using resistive heater - B01L 2400/0409: centrifugal forces - G01N 2021/1765: {Method using an image detector and processing of image signal} - G01N 2021/3155: {Measuring in two spectral ranges, e.g. UV and visible} - G01N 2021/6417: {Spectrofluorimetric devices} - G01N 2021/6439: {with indicators, stains, dyes, tags, labels, marks} - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES ## Abstract Provided herein are systems, devices, and methods for performing optical multianalyte detection in a sample, such as a human blood sample. The system processes sample in a single-use support pack that holds the blood sample and contains pipette tips, buffers, reagent preparation wells and a monolayer. Sample is transferred from the sample pack to a single-use disc consumable that contains microwells pre-filled with dried chemistries, plasma separation features, and a hematocrit channel. The instrument comprises a cell imager, an absorbance module comprising a spectrophotometer, a fluorescent laser scanning module, and a camera. The system uses only a small amount of blood from a single heparinized sample, to simultaneously provide results for a full panel of routine blood tests spanning elecochemistry, clinical chemistry, hematology, immunoassays and combinations thereof. ## Claims ### Claim 1 An optical method for detecting the presence of a target analyte in a sample, the method comprising: a) moving automatically a first portion of a whole blood sample from a support pack to a first well in a disk in a detection instrument and moving automatically a second portion of the whole blood sample from the support pack to a reaction well in the support pack, the disk comprising dried chemistries for processing the sample, plasma separation features configured to separate whole blood into plasma, and a hematocrit channel configured to execute the hematocrit assay, the support pack comprising the whole blood sample, pipette tips, buffers, a reagent for processing the sample, reagent preparation wells, and a monolayer assay device configured to operate as a hematology slide; b) separating by the detection instrument plasma from the first portion of the whole blood sample in the disk while simultaneously moving reagent in the support pack for processing the plasma generated in the disk and diluting the second portion of the whole blood sample in the support pack; c) moving automatically by the detection instrument the plasma generated from the first portion of the whole blood sample from the disk to the support pack; d) diluting by the detection instrument the plasma generated from the first portion of the whole blood sample in the support pack; e) moving automatically by the detection instrument the plasma generated from the first portion of the whole blood sample from the support pack to the disk; and f) optically detecting by the detection instrument the presence or absence of said first target analyte, the detection instrument comprising a laser and at least one of a spectrophotometer, or a camera. ### Claim 2 (canceled) ### Claim 3 (depends on 1) The optical method of claim 1, further comprising moving a third portion of the whole blood sample from the support pack to a second well in the disk; separating by the detection instrument, plasma from the third portion of the whole blood sample in the disk and taking an image by a second camera associated with the disk prior to detecting the presence or absence of the first target analyte in the disk. ### Claim 4 An optical method for detecting the presence of a target analyte in a sample, the method comprising: a) moving automatically a first portion of a whole blood sample from a support pack to a first well in a disk in a detection instrument, the disk comprising dried chemistries for processing the sample, plasma separation features configured to separate whole blood into plasma, and a hematocrit channel configured to execute the hematocrit assay, the support pack comprising the whole blood sample, pipette tips, buffers, a reagent for processing the sample, reagent preparation wells, and a monolayer assay device configured to operate as a hematology slide: b) separating by the detection instrument plasma from the first portion of the whole blood sample in the disk while simultaneously moving reagent in the support pack for processing the plasma generated in the disk: c) moving automatically by the detection instrument the plasma generated from the first portion of the whole blood sample from the disk to the support pack: d) diluting by the detection instrument the plasma generated from the first portion of the whole blood sample in the support pack: e) moving automatically by the detection instrument the plasma generated from the first portion of the whole blood sample from the support pack to the disk: f) optically detecting by the detection instrument the presence or absence of said first target analyte, the detection instrument comprising a laser and at least one of a spectrophotometer, or a camera: further comprising moving by the detection instrument a third portion of the whole blood sample from the support pack to a second well in the disk; hybridizing by the detection instrument a probe to a second target analyte in the second well to form a bead-antigen-fluorescent complex; moving by the detection instrument the bead-antigen- fluorescent complex to the support pack; washing automatically by the detection instrument the bead-antigen-fluorescent complex in the support pack; moving the washed bead-antigen-fluorescent complex back to the disk to a third well wherein the second well and the third well are different; and detecting by the detection instrument the presence or absence of said second target analyte in the disk after detecting by the detection instrument the presence or absence of said first target analyte in the disk. ### Claim 5 (depends on 3) The optical method of claim 3, further comprising counting the number of cells in the sample comprising moving a fourth portion of the whole blood sample from the support pack to a third well in the disk, separating by the detection instrument the fourth portion of the whole blood sample into plasma in the disk, moving the plasma generated from the fourth portion of the whole blood sample from the disk to the monolayer assay device in the support pack and detecting the number of cells in the plasma generated from the fourth portion of the whole blood sample in the support pack after optically detecting by the detection instrument the presence or absence of said first target analyte in the first portion of the whole blood sample in the disk. ### Claim 6 (depends on 1) The optical method of claim 1, further comprising: automatically calibrating the detection instrument based on a barcode on the support pack, the barcode comprising information for the assay; collecting by the disk and support pack all waste, generated during analysis of the sample; determining the location of the support pack by a barcode scanner, the barcode scanner operatively coupled to a second processor and associated with the support pack prior to moving the first portion of the whole blood sample from the support pack to the first well in the disk; aligning the support pack and the spectrophotometer, or the camera by the detection instrument using a first rail associated with a support pack tray the support pack tray holding the support pack and a second rail associated with the spectrophotometer, or the camera prior to moving the first portion of the whole blood sample, wherein the detection instrument is not connected to the internet. ### Claim at position 7 -20. (canceled) ### Claim 21 An optical method for detecting the presence of target analyte in a sample, the method comprising: a) receiving by a detection instrument a first cartridge comprising dried chemistries, plasma separation features, and a hematocrit channel; b) receiving by the detection instrument a second cartridge comprising the whole blood sample, pipette tips, buffers, reagent preparation wells, and a monolayer assay device; c) moving automatically by the detection instrument a first portion of a whole blood sample from the second cartridge to a first well in the first cartridge and moving automatically by the detection instrument a second portion of a whole blood sample from the second cartridge to a reaction well in the second cartridge; d) moving automatically by the detection instrument a reagent from the second cartridge to the first well in the first cartridge; e) and diluting the second portion of the whole blood sample in the second cartridge; and f optically detecting by the detection instrument the presence or absence of a first target analyte in the first portion, the instrument comprising a laser and at least one of a spectrophotometer, and a camera. ### Claim at position 9 -30. (canceled) ### Claim 31 An optical method for detecting the presence of target analyte in a sample, the method comprising: a) moving automatically a first portion of a whole blood sample from a second cartridge to a first well in a first cartridge in a detection instrument, the first cartridge comprising dried chemistries, plasma separation features, and a hematocrit channel, the second cartridge comprising the whole blood sample, pipette tips, buffers, reagent preparation wells, and a monolayer assay device; b) moving reagent from the second cartridge to the first well in the first cartridge; c) optically detecting by the detection instrument the presence or absence of a first target analyte in the first well, the detection instrument comprising a laser and at least one of a spectrophotometer or a camera; (d) moving automatically a second portion of the whole blood sample from the second cartridge to a first tube in the second cartridge in a detection instrument; (e) moving automatically a buffer from the second cartridge to the first tube in the second cartridge; (f) optically detecting by the detection instrument the presence or absence of a second target analyte in the first tube. ### Claim at position 11 -34. (canceled) ### Claim 35 (depends on 4) The method of claim 4, further comprising mixing by the detection instrument the bead-antigen-fluorescent complex in the disk by rotating the disk clockwise and counterclockwise and moving by the detection instrument the reagent for processing the plasma generated in the disk by aspirating with a pipette. ### Claim 36 (depends on 1) The method of claim 1, further comprising receiving sequentially by the detection instrument the support pack supported by a support pack tray and associated with a first rail and the disk supported by a disk tray and associated with a second rail. ### Claim 37 An optical method for detecting the presence of a target analyte in a sample, the method comprising: a) moving automatically a first portion of a whole blood sample from a support pack to a first well in a disk in a detection instrument, the disk comprising dried chemistries for processing the sample, plasma separation features configured to separate whole blood into plasma, and a hematocrit channel configured to execute the hematocrit assay, the support pack comprising the whole blood sample, pipette tips, buffers, a reagent for processing the sample, reagent preparation wells, and a monolayer assay device configured to operate as a hematology slide: b) separating by the detection instrument plasma from the first portion of the whole blood sample in the disk while simultaneously moving reagent in the support pack for processing the plasma generated in the disk: c) moving automatically by the detection instrument the plasma generated from the first portion of the whole blood sample from the disk to the support pack: d) diluting by the detection instrument the plasma generated from the first portion of the whole blood sample in the support pack: e) moving automatically by the detection instrument the plasma generated from the first portion of the whole blood sample from the support pack to the disk: f) optically detecting by the detection instrument the presence or absence of said first target analyte, the detection instrument comprising a laser and at least one of a spectrophotometer, or a camera; and further comprising optically detecting by the detection instrument the presence or absence of said first target analyte in the first portion of the whole blood sample in the disk while simultaneously optically detecting by the detection instrument the presence or absence of an additional target analyte in an additional portion of the whole blood sample in the support pack. ### Claim 38 (depends on 1) The optical method of claim 1, wherein optically detecting by the detection instrument is by the camera aligned with the disk the presence, absence, or value of the first target analyte wherein the first target analyte is selected from the group comprising Glucose, BUN, Creatinine, Calcium, Protein, Total, Albumin, Bilirubin, Total, Alkaline Phosphatase, AST (SGOT), ALT (SGPT), Cholesterol Total, Triglycerides, HDL Cholesterol, Hemoglobin, or Hemoglobin Alc. ### Claim 39 (depends on 38) The optical method of claim 38, further comprising optically detecting by the detection instrument by a second optical detector aligned with the disk the presence or absence of a third target analyte wherein the third target analyte is TSH (Thyroid Stimulating Hormone). ### Claim 40 (depends on 1) The optical method of claim 1, further comprising detecting by a third detector aligned with the support pack the value of a third target analyte in the second portion of the whole blood sample wherein the third target analyte is WBC, RBC, Platelets, Neutrophils (%), Lymphocytes (%), Neutrophils (Absolute), or Lymphocytes (Absolute). ### Claim 41 (depends on 40) The optical method of claim 40, wherein detecting by the detection instrument by a third detector aligned with the support pack occurs at the same time as detecting by a first detector aligned with the disk. ### Claim 42 (depends on 40) The optical method of claim 40, wherein detecting by the detection instrument by a third detector aligned with the support pack occurs at the same time as detecting the first target analyte by the camera aligned with the disk. ### Claim 43 (depends on 4) The optical method of claim 4, further comprising, moving automatically a second portion of the whole blood sample from the support pack to a second well in the disk; separating by the detection instrument plasma from the second portion of the whole blood sample in the disk; moving automatically by the detection instrument the plasma generated from the second portion of the whole blood sample from the disk back to the support pack; incubating by the detection instrument the plasma generated from the second portion of the whole blood sample in the support pack; detecting a third target analyte in the plasma generated from the third second portion of the whole blood sample by a third detector aligned with the support pack wherein detecting by a third detector aligned with the support pack cannot occur at the same time as washing automatically the bead-antigen-fluorescent complex in the support pack. ### Claim 44 (canceled) ### Claim 45 (depends on 1) The optical method of claim 1, wherein the reagent is an immunoassay wash buffer. ### Claim 46 (depends on 4) The method of claim 4, wherein the spectrophotometer detects the second target analyte. ### Claim 47 (depends on 4) The method of claim 4, further comprising moving a second portion of the whole blood sample from the support pack to a reaction well in the support pack and simultaneous to optically detecting by the detection instrument the presence or absence of said first target analyte in the first portion of the whole blood sample in the disk, processing the second portion of the whole blood sample in the support pack.