# US 2025/0085254 A1 · Sensor assembly, system, method and ph sensor > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2025/0085254 A1 - Document ID: US20250085254A1 - Status: Published application - Kind: A1 - Application number: 18/717,282 - Filed: 2022-12-06 - Published: 2025-03-13 - Earliest claimed: 2021-12-06 - Assignees: Analog Devices, Inc. - Applicants: Analog Devices, Inc. - Inventors: Hari CHAUHAN; Alexander C. STANGE; Mohamed AZIZE - Web page: https://patentgrep.com/patent/US20250085254A1 ## CPC - G01N 27/4145: {specially adapted for biomolecules, e.g. gate electrode with immobilised receptors} - G01N 27/125: {Composition of the body, e.g. the composition of its sensitive layer} - G01N 27/30: Electrodes, e.g. test electrodes; Half-cells (G01N27/414 takes precedence) - G01N 27/3276: {being a hybridisation with immobilised receptors (using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68)} - G01N 27/4146: {involving nanosized elements, e.g. nanotubes, nanowires} - B82Y 15/00: Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES ## Abstract The present disclosure provides a sensor assembly for detecting the presence of at least one amplification product of an isothermal amplification process (i.e., an isothermal nucleic acid amplification process). The sensor assembly includes a first sensor responsive to the detection of an indicator of the presence of at least one amplification product to provide a first signal, the first sensor comprising a sensing surface arranged to contact a sample in the amplification product receiving region, the sensing surface comprises a first layer comprising a one-dimensional or two-dimensional material. Alternatively or additionally, the disclosure provides a pH sensor comprising a sensing surface comprising a first layer comprising a one-dimensional or two-dimensional material. ## Claims ### Claim 1 A sensor assembly for detecting the presence of at least one amplification product of an isothermal amplification process, the sensor assembly comprising: an amplification product receiving region; and a first sensor responsive to the detection of an indicator of the presence of at least one amplification product to provide a first signal, the first sensor comprising a sensing surface arranged to contact a sample in the amplification product receiving region, wherein the sensing surface comprises a first layer comprising a one-dimensional or two-dimensional material; and wherein the first sensor is either (i) a pH sensor adapted to detect a change in pH indicative of the presence of at least one amplification product; or (ii) a sensor adapted to detect the presence of at least one amplification product directly. ### Claim 2 (depends on 1) The sensor assembly of claim 1, wherein the one-dimensional or two-dimensional material is selected from graphene, hexagonal boron-nitride, carbon nano-tubes, or a combination thereof. ### Claim 3 (depends on 2) The sensor assembly of claim 2, wherein the one-dimensional or two-dimensional material is single-walled carbon nanotubes. ### Claim 4 (depends on 1) The sensor assembly of claim 1, wherein the first layer is formed of the one-dimensional or two-dimensional material. ### Claim 5 (depends on 1, 2, 3, 4) The sensor assembly of any one of claims 1 to 4, wherein the first sensor is an electrode-based sensor comprising at least one electrode adapted to provide the first signal. ### Claim 6 (depends on 1, 2, 3, 4) The sensor assembly of any one of claims 1 to 4, wherein the first sensor comprises a field effect transistor (FET) adapted to provide the first signal, and wherein the first layer is provided on a surface of the FET. ### Claim 7 (depends on 6) The sensor assembly of claim 6, wherein the first sensor further comprises a second layer beneath a channel of the field effect transistor, the second layer comprising or being formed of a one dimensional or two-dimensional material. ### Claim 8 (depends on 6) The sensor assembly of claim 6, wherein the first layer comprises or is formed of hexagonal-boron nitride. ### Claim 9 (depends on 6) The sensor assembly of claim 6, wherein the FET is a graphene-FET such that the channel is a graphene channel, and the first layer is a hexagonal boron nitride layer formed on the channel of the FET. ### Claim 10 (depends on 1, 2, 3, 4) The sensor assembly of any one of claims 1 to 4, further comprising a second sensor responsive to the detection of an indicator of the presence of at least one amplification product to provide a second signal; and wherein the first sensor is a pH sensor adapted to detect a change in pH indicative of the presence of at least one amplification product and the second sensor is a sensor adapted to detect the presence of at least one amplification product directly. ### Claim 11 (depends on 1, 2, 3, 4) The sensor assembly of any one of claims 1 to 4, further comprising an optical sensor adapted to detect a change in optical properties corresponding to the presence of at least one amplification product. ### Claim 12 (depends on 1, 2, 3, 4) The sensor assembly of any one of claims 1 to 4, further comprising an ion selective membrane provided on the sensing surface. ### Claim 13 A system for isothermal amplification of a sample, the system comprising: an isothermal amplification assembly for carrying out an isothermal amplification process on the sample to produce at least one amplification product; and a sensor assembly for detecting the presence of the at least one amplification product, the sensor assembly comprising: an amplification product receiving region; and a first sensor responsive to the detection of indicator of the presence of at least one amplification product to provide a first signal, the first sensor comprising a sensing surface arranged to contact a sample in the amplification product receiving region, wherein the sensing surface comprises a first layer comprising a one-dimensional or two-dimensional material. ### Claim 14 (depends on 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13) The system of claim 13, wherein the sensor assembly is a sensor assembly according to any of claims 1 to 12. ### Claim 15 (depends on 14) The system of claim 14, wherein the one-dimensional or two-dimensional material is selected from graphene, hexagonal boron-nitride, carbon nano-tubes, or a combination thereof. ### Claim 16 (depends on 13, 14, 15) The system of any one of claims 13 to 15, wherein the one-dimensional or two-dimensional material is single-walled carbon nanotubes. ### Claim 17 (depends on 13, 14, 15) The system of any one of claims 13 to 15, wherein the first sensor is (i) a pH sensor adapted to detect a change in pH indicative of the presence of at least one amplification product; or (ii) a sensor adapted to detect the presence of at least one amplification product directly. ### Claim 18 (depends on 13, 14, 15) The system of any one of claims 13 to 15, further comprising a second sensor responsive to the detection of an indicator of the presence of at least one amplification product to provide a second signal; and wherein the first sensor is a pH sensor adapted to detect a change in pH indicative of the presence of at least one amplification product and the second sensor is a sensor adapted to detect the presence of at least one amplification product directly. ### Claim 19 (depends on 13, 14, 15) The system of any one of claims 13 to 15, wherein the isothermal amplification assembly is a loop assisted isothermal amplification (LAMP) assembly. ### Claim 20 A method for determining the presence of an analyte in a sample, the method comprising: subjecting the sample to an isothermal amplification process, the isothermal amplification process producing at least one amplification product in the event that the analyte is present; providing the at least one amplification product to an amplification product receiving region; and detecting the presence of the at least one amplification product using a sensor assembly, the sensor assembly comprising a first sensor responsive to the detection of indicator of the presence of at least one amplification product to provide a first signal, the first sensor comprising a sensing surface arranged to contact a sample in the amplification product receiving region, wherein the sensing surface comprises a first layer comprising a one-dimensional or two-dimensional material. ### Claim 21 (depends on 20) The method of claim 20, further comprising: determining the presence of the at least one amplification product using a second sensor. ### Claim 22 (depends on 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 20, 21) The method of claim 20 or claim 21, wherein the sensor assembly is the sensor assembly according to any of claims 1 to 12. ### Claim 23 A pH sensor, comprising: a sensing surface arranged to contact a sample, the sensing surface comprising a first layer comprising a one-dimensional or two-dimensional material, wherein pH sensor is adapted to provide a signal indicative of the pH of a sample. ### Claim 24 (depends on 23) The pH sensor of claim 23, wherein the one-dimensional or two-dimensional material is selected from graphene, hexagonal boron-nitride, carbon nano-tubes, or a combination thereof. ### Claim 25 (depends on 23) The pH sensor of claim 23, wherein the one-dimensional or two-dimensional material is single-walled carbon nano-tubes. ### Claim 26 (depends on 23, 24, 25) The pH sensor of any one of claims 23 to 25, wherein the first layer is formed of the one-dimensional or two-dimensional material. ### Claim 27 (depends on 23, 24, 25) The pH sensor of any one of claims 23 to 25, wherein the first sensor an electrode-based sensor comprising at least one electrode adapted to provide the first signal. ### Claim 28 (depends on 23, 24, 25) The pH sensor of any one of claims 23 to 25, wherein the first sensor comprises a field effect transistor adapted to provide the first signal, and wherein the first layer is provided on a surface of the field effect transistor.