# US 2025/0085223 A1 · Analysis method, analysis apparatus, and analysis kit for calculating concentration of target substance

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2025/0085223 A1
- Document ID: US20250085223A1
- Status: Published application
- Kind: A1
- Application number: 18/813,299
- Filed: 2024-08-23
- Published: 2025-03-13
- Earliest claimed: 2023-09-11
- Assignees: Canon Kabushiki Kaisha; Canon Medical Systems Corporation
- Applicants: Canon Kabushiki Kaisha; Canon Medical Systems Corporation
- Inventors: NORISHIGE KAKEGAWA; TAKAHIRO MASUMURA; TEIGO SAKAKIBARA; YUYA KOMORI
- Web page: https://patentgrep.com/patent/US20250085223A1

## CPC

- G01N 21/6428: {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53)}
- B01L 3/508: {Rigid containers without fluid transport within}
- G01N 15/0211: {Investigating a scatter or diffraction pattern}
- G01N 33/53: Immunoassay; Biospecific binding assay; Materials therefor
- B01L 2200/16: Reagents, handling or storing thereof
- B01L 2300/0663: Whole sensors
- B01L 2300/0832: cylindrical, tube shaped
- B01L 2300/12: Specific details about materials
- G01N 21/6445: {Measuring fluorescence polarisation}
- G01N 2015/0053: {in liquids, e.g. trouble}
- G01N 2015/0222: {from dynamic light scattering, e.g. photon correlation spectroscopy}
- G01N 2021/7786: {Fluorescence}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

Provided is an analysis method for determining at least any one of: presence or absence of a target substance; and a concentration of the target substance through use of a reagent that reacts with the target substance, the analysis method including: a loading step of loading: a sample containing the target substance; a hydrophilic polymer that reacts with a silanol group of a glass vessel; and the reagent into the glass vessel; a reaction step of causing the target substance and the reagent to react with each other to provide a reaction liquid; and an analysis step of determining at least any one of: the presence or absence of the target substance; and the concentration of the target substance in the reaction liquid, wherein the loading step includes loading the reagent after or simultaneously with the loading of the hydrophilic polymer.

## Claims

### Claim 1

An analysis method for determining at least any one of: presence or absence of a target substance; and a concentration of the target substance through use of a reagent that reacts with the target substance, the analysis method comprising:
a loading step of loading: a sample containing the target substance; a hydrophilic polymer that reacts with a silanol group of a glass vessel; and the reagent into the glass vessel;
a reaction step of causing the target substance and the reagent to react with each other to provide a reaction liquid; and
an analysis step of determining at least any one of: the presence or absence of the target substance; and the concentration of the target substance in the reaction liquid,
wherein the reagent includes a luminescent reagent having a hydrophilic surface, and the luminescent reagent includes a luminescent particle, and
wherein the loading step includes loading the reagent after or simultaneously with the loading of the hydrophilic polymer.

### Claim 2 (depends on 1)

The analysis method according to claim 1, wherein the analysis step includes determining at least any one of: the presence or absence of the target substance; and the concentration of the target substance in the reaction liquid by measuring a value (R) for polarization anisotropy of the reaction liquid.

### Claim 3 (depends on 2)

The analysis method according to claim 2, wherein the following formula is satisfied: R0≥0.01, where R0 represents the R measured for the reagent unreacted with the target substance.

### Claim 4 (depends on 2)

The analysis method according to claim 2, wherein the R is defined to be “r” in the following equation (1):
r
=
I
vv
-
G
*
I
vH
I
vv
+
2
*
G
*
I
vH
⁢
G
=
I
Hv
I
HH
(
1
)
in the equation (1),
IVV represents a luminescence intensity of a luminescence component having a vibration direction parallel to that of a first polarized light beam at a time of excitation by the first polarized light beam,
IVH represents a luminescence intensity of a luminescence component having a vibration direction orthogonal to that of the first polarized light beam at the time of excitation by the first polarized light beam,
IHV represents a luminescence intensity of a luminescence component having a vibration direction orthogonal to that of a second polarized light beam having a vibration direction orthogonal to that of the first polarized light beam at a time of excitation by the second polarized light beam,
IHH represents a luminescence intensity of a luminescence component having a vibration direction parallel to that of the second polarized light beam having a vibration direction orthogonal to that of the first polarized light beam at the time of excitation by the second polarized light beam, and
G represents a correction value.

### Claim 5 (depends on 1)

The analysis method according to claim 1, wherein the hydrophilic polymer forms a hydrogen bond with the silanol group of the glass vessel.

### Claim 6 (depends on 1)

The analysis method according to claim 1, wherein a concentration of the luminescent reagent in the reaction liquid is 0.0001 mg/ml or more and 0.1 mg/ml or less.

### Claim 7 (depends on 1)

The analysis method according to claim 1, wherein the hydrophilic polymer includes polyvinylpyrrolidone.

### Claim 8 (depends on 1)

The analysis method according to claim 1, wherein the luminescent particle contains a europium complex.

### Claim 9 (depends on 1)

The analysis method according to claim 1, wherein the luminescent reagent contains a ligand that binds to the target substance.

### Claim 10 (depends on 9)

The analysis method according to claim 9, wherein the ligand is an antibody, and the target substance is an antigen.

### Claim 11

An analysis apparatus for determining at least any one of: presence or absence of a target substance; and a concentration of the target substance, the analysis apparatus comprising:
a glass vessel to be used for accommodating a reaction liquid containing: a sample containing the target substance; and a reagent that reacts with the target substance;
a unit configured to load the sample containing the target substance into the glass vessel;
a unit configured to load a hydrophilic polymer that reacts with a silanol group of the glass vessel into the glass vessel;
a unit configured to load the reagent into the glass vessel after or simultaneously with the loading of the hydrophilic polymer;
an irradiation unit configured to irradiate, as irradiation light, the glass vessel with light that is linearly polarized light;
a measurement unit configured to measure fluorescence intensities of fluorescence of two polarized light components in a parallel direction and an orthogonal direction with respect to the linearly polarized light; and
a processing unit configured to process results obtained by the measurement unit, to thereby determine at least any one of: the presence or absence of the target substance; and the concentration of the target substance in the reaction liquid,
wherein the reagent includes a luminescent reagent having a hydrophilic surface, and the luminescent reagent includes a luminescent particle.

### Claim 12

An analysis kit to be used in an analysis method for determining at least any one of: presence or absence of a target substance; and a concentration of the target substance based on polarization anisotropy, the analysis kit comprising:
a reagent that reacts with the target substance;
a glass vessel to be used for accommodating a reaction liquid containing: a sample containing the target substance; and the reagent; and
a hydrophilic polymer that reacts with a silanol group of the glass vessel,
wherein the reagent includes a luminescent reagent having a hydrophilic surface, and the luminescent reagent includes a luminescent particle.