# US 2025/0085209 A1 · Flow cytometer and particle detection method > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2025/0085209 A1 - Document ID: US20250085209A1 - Status: Published application - Kind: A1 - Application number: 18/960,168 - Filed: 2024-11-26 - Published: 2025-03-13 - Earliest claimed: 2017-03-30 - Assignees: Sysmex Corporation - Applicants: Sysmex Corporation - Inventors: Motoi KINISHI; Shohei KAMURA; Hiroo TATSUTANI; Takashi YOSHIOKA; Takayuki NAKAJIMA - Web page: https://patentgrep.com/patent/US20250085209A1 ## CPC - G01N 15/1425: {using an analyser being characterised by its control arrangement} - G01N 15/1404: Handling flow, e.g. hydrodynamic focusing - G01N 15/1434: Optical arrangements - G01N 15/1436: {the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell} - G01N 35/00871: {Communications between instruments or with remote terminals} - G01N 2035/00891: {Displaying information to the operator} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract A general-purpose flow cytometer capable for using a plurality of combinations of fluorescent-labeled antibodies is provided. The flow cytometer includes a flow cell to flow therein a measurement sample prepared from a sample and a labeling reagent; light sources configured to irradiate with light; light receiving elements including first and second light receiving elements, the first light receiving element receiving first fluorescence from the particles that are labeled with a first fluorescent-labeled antibody and the second light receiving element receiving second fluorescence from the particles that are labeled with a second fluorescent-labeled antibody; and a storage configured to store a measurement condition associated with a measurement item corresponding to a predetermined combination of the fluorescent-labeled antibodies among the plurality of combinations of the fluorescent-labeled antibodies; and a processor programmed to execute operations in response to receiving instruction of measurement of identified measurement item. ## Claims ### Claim 1 A general-purpose flow cytometer capable for using a plurality of combinations of fluorescent-labeled antibodies, comprising: a flow cell to flow therein a measurement sample prepared from a sample and a labeling reagent, wherein the sample contains particles and the labeling reagent contains a combination of the fluorescent-labeled antibodies to label antigens on the particles; light sources configured to irradiate with light, the particles in the measurement sample flowing in the flow cell; light receiving elements including first and second light receiving elements, the first light receiving element receiving first fluorescence from the particles that are labeled with a first fluorescent-labeled antibody and the second light receiving element receiving second fluorescence from the particles that are labeled with a second fluorescent-labeled antibody; a storage configured to store a measurement condition associated with a measurement item corresponding to a predetermined combination of the fluorescent-labeled antibodies among the plurality of combinations of the fluorescent-labeled antibodies, wherein the measurement condition includes at least: (1) detection sensitivity of the light receiving elements; and (2) fluorescence correction defining (2a) the second fluorescence that is received by the first light receiving element and to be canceled for a first detection signal obtainable from the fluorescence received by the first light receiving element and (2b) the first fluorescence that is received by the second light receiving element and to be canceled for a second detection signal obtainable from the second fluorescence received by the second light receiving element, and a processor programmed to execute operations in response to receiving instruction of measurement of identified measurement item, the operations including: reading out, from the storage, the measurement condition associated with the identified measurement item; irradiating with the light, by the light sources, the measurement sample flowing in the flow cell; obtaining, the first detection signal and the second detection signal, with the detection sensitivity adjusted according to the measurement condition; correcting the first detection signal and the second detection signal with the fluorescence correction according to the measurement condition; and creating a scattergram based on the corrected first detection signal and the corrected second detection signal according to the measurement condition. ### Claim 2 (depends on 1) The flow cytometer according to claim 1, wherein the detection sensitivity includes a value indicating an amplification degree of an output voltage of the respective light receiving elements and a voltage value applied to the respective light receiving elements. ### Claim 3 (depends on 1) The flow cytometer according to claim 1, wherein the fluorescence correction includes a light wavelength distribution amount outside a detection target included in each of the first detection signal and the second detection signal. ### Claim 4 (depends on 1) The flow cytometer according to claim 1, wherein the measurement condition further includes gating information defining parameters of the scattergram to plot thereon the particles based on the first detection signal and the second detection signal, and the creating the scattergram is based on the gating information, the corrected first detection signal and the corrected second detection signal according to the measurement condition. ### Claim 5 (depends on 4) The flow cytometer according to claim 4, wherein the gating information includes an area for selecting specific particles on a distribution map of optical information derived from the particles. ### Claim 6 (depends on 1) The flow cytometer according to claim 1, wherein the first detection signal is related to the first fluorescence derived from the particles included in the measurement sample and the second detection signal is related to the second fluorescence derived from the particles included in the measurement sample. ### Claim 7 (depends on 1) The flow cytometer according to claim 1, wherein the light receiving elements further comprise a third light receiving element receiving third fluorescence from the particles that are labeled with a third fluorescent-labeled antibody. ### Claim 8 (depends on 7) The flow cytometer according to claim 7, wherein the light receiving elements further comprise a fourth light receiving element receiving forward scattered light and a fifth light receiving element receiving side scattered light. ### Claim 9 (depends on 1) The flow cytometer according to claim 1, wherein the measurement item is related to a human immunodeficiency virus. ### Claim 10 (depends on 1) The flow cytometer according to claim 1, further comprising a suction tube configured to suction the measurement sample from a sample container that contains the measurement sample; wherein the light receiving elements are configured to detect fluorescence derived from the respective fluorescent-labeled antibodies contained in the measurement sample suctioned by the suction tube. ### Claim 11 (depends on 1) The flow cytometer according to claim 1, wherein the storage is configured to store a correction of the measurement condition received from a user.