# US 2025/0085200 A1 · Tissue Cutting Assembly And Associated Method Of Use > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2025/0085200 A1 - Document ID: US20250085200A1 - Status: Published application - Kind: A1 - Application number: 18/828,589 - Filed: 2024-09-09 - Published: 2025-03-13 - Earliest claimed: 2023-09-12 - Inventors: Joseph Richard Cant; Gary Michael Wilson - Web page: https://patentgrep.com/patent/US20250085200A1 ## CPC - G01N 1/286: {involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising (microtomes G01N1/06; pulverising in general B02C; mixing in general B01F)} - G01N 1/36: Embedding or analogous mounting of samples - G01N 2001/2873: {Cutting or cleaving} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract A tissue cutting assembly used for cutting tissue samples from a tissue sample includes a substrate configured to receive the tissue sample thereon, a frame defining an opening through which the tissue sample is exposed, and a tissue cutting guide positioned and held stationary within the opening in at least two distinct positions onto the tissue sample. The tissue cutting guide defines one or more cutting slits through which a user can insert and guide a cutting device to cut the tissue sample along a length corresponding to the length of the one or more slits in each of the at least two distinct positions to form cut tissue samples of desired widths and lengths. ## Claims ### Claim 1 A tissue cutting assembly comprising: a substrate configured to receive a tissue sample thereon; a frame secured to said substrate having an internal perimeter defining an opening; and a tissue cutting guide having an external perimeter complimentary in configuration to said internal perimeter of said frame, said tissue cutting guide defining a plurality of spaced apart slits extending therethrough; wherein said tissue cutting guide is positionable within said opening of said frame in any one of a plurality of positions relative to said frame, and wherein said external perimeter of said tissue cutting guide at least partially abuts said internal perimeter of said frame to prevent rotational movement of said tissue cutting guide relative to said frame and said substrate once placed in said one position of said plurality of positions for allowing a user to apply desired cuts to the tissue sample through the spaced apart slits. ### Claim 2 (depends on 1) The tissue cutting assembly of claim 1, wherein a length of one of said plurality of spaced apart slits extends transverse to said length of said same one of said plurality of slits when said tissue cutting guide is moved from a first position of said plurality of positions to a second position of said plurality of positions, said second position different than said first position. ### Claim 3 (depends on 1) The tissue cutting assembly of claim 1, wherein said plurality of spaced apart slits are parallel to each other. ### Claim 4 (depends on 1) The tissue cutting assembly of claim 1, wherein a length of said one of said plurality of spaced apart slits extends transverse to a length of at least an other one of said plurality of slits. ### Claim 5 (depends on 1) The tissue cutting assembly of claim 1, wherein one or more of said plurality of slits is not parallel to at least one other one of said plurality of slits. ### Claim 6 (depends on 1) The tissue cutting assembly of claim 1, wherein said inner surface of said frame is a polygonal-shaped inner surface and said outer surface of said tissue cutting guide is a polygonal-shaped outer surface complimentary to said polygonal-shaped inner surface. ### Claim 7 (depends on 1) The tissue cutting assembly of claim 1, wherein said inner surface of said frame is a regular polygonal-shaped inner surface and said outer surface of said tissue cutting guide is a regular polygonal-shaped outer surface complimentary to said regular polygonal-shaped inner surface. ### Claim 8 (depends on 7) The tissue cutting assembly of claim 7, wherein said inner surface of said frame is a square-shaped inner surface and said outer surface of said tissue cutting guide is a square-shaped outer surface complimentary to said square-shaped inner surface, and wherein said plurality of positions comprises a first position and a second position different than said first position with said tissue cutting guide rotated 90 degrees and positioned within said opening of said frame in said second position relative to said first position. ### Claim 9 (depends on 8) The tissue cutting assembly of claim 8, wherein said plurality of spaced apart slits are parallel to each other. ### Claim 10 (depends on 1) The tissue cutting assembly of claim 1, wherein said frame includes a notch positioned adjacent to said internal perimeter for allowing a finger of a user to engage said external perimeter of said tissue cutting guide to remove said tissue cutting guide from within said opening. ### Claim 11 (depends on 1) The tissue cutting assembly of claim 1, wherein a width between any two adjacent slits of said plurality of slits is the same. ### Claim 12 (depends on 1) The tissue cutting assembly of claim 1, wherein a width between any two adjacent slits of said plurality of slits is different than a width at least one of pair of adjacent slits of said plurality of slits. ### Claim 13 (depends on 1) The tissue cutting assembly of claim 1, wherein a length of each of said plurality of slits is the same. ### Claim 14 (depends on 1) The tissue cutting assembly of claim 1, wherein a length of said one of said plurality of slits is different than a length of at least one other one of said plurality of slits. ### Claim 15 (depends on 1) The tissue cutting assembly of claim 1, wherein said frame includes a plurality of notches disposed within said internal perimeter of said frame to allow access to said external perimeter of said tissue cutting guide to facilitate removal of said tissue cutting guide from within said opening of said frame. ### Claim 16 A method of preparing desired sized and shaped tissue samples from a tissue sample using a frame, a tissue cutting guide, and a substrate, the frame having an inner perimeter defining an opening, and the tissue cutting guide having an external perimeter complimentary in configuration to the internal perimeter and defining a plurality of slits extending therethrough, said method comprising the steps of: disposing the tissue sample on the substrate; disposing the frame on the substrate such that the tissue sample is held stationary by the frame and such that a portion of the tissue sample is visible and accessible through the opening; securing the frame to the substrate; disposing the tissue cutting guide within the opening of the frame in any one of a plurality of positions relative to the frame, wherein the external perimeter of the tissue cutting guide at least partially abuts the internal perimeter of the frame to prevent rotational movement of the tissue cutting guide relative to the frame and the substrate; and allowing a user to apply desired cuts to the tissue sample through the spaced apart slits. ### Claim 17 (depends on 16) The method of claim 16, wherein said step of allowing a user to apply desired cuts to the tissue sample comprises the steps of: (a) inserting a blade of a cutting device within one of the slits of the plurality of slits; and (b) moving the blade of the cutting device along the length of the one slit to cut the tissue sample. ### Claim 18 (depends on 17) The method of claim 17, wherein said step of allowing a user to apply desired cuts to the tissue sample further comprises the steps of: (c) removing the blade of the cutting device from within the one slit; and (d) optionally repeating steps (a)-(c) for one or more additional slits of the plurality of slits. ### Claim 19 (depends on 17) The method of claim 17, further comprising the steps of: removing the tissue cutting guide from within the opening of the frame; rotating the tissue cutting guide after the tissue cutting guide is removed from the frame; disposing the tissue cutting guide within the opening of the frame in a second position of the plurality of positions relative to the frame, the second position different than a first position, wherein a length of one of the plurality of slits extends transverse to the length of the same one of the plurality of slits when the tissue cutting guide is moved from the first position to the second position; and cutting the tissue sample while the tissue cutting guide is in the second position. ### Claim 20 (depends on 19, 20) The method of claim 19, wherein said step of cutting the tissue sample while the tissue cutting guide is in the second position comprises the steps of: (e) inserting a blade of a cutting device within one of the slits of the plurality of slits; and (f) moving the blade of the cutting device along the length of the one slit to cut the tissue sample. 21 The method of claim 20, wherein the step of cutting the tissue sample while the tissue cutting guide is in the second position further comprises the steps of: (g) removing the blade of the cutting device from within the one slit; and (h) optionally repeating steps (e)-(f) for one or more additional slits of the plurality of slits.