Carbon Fixation Pathway

A method of carbon fixation without rubisco, comprising providing carbon dioxide or bicarbonate to a system comprising an engineered pathway configured to incorporate bicarbonate into fructose-6-phosphate and comprising recombinant enzymes:

• i) Hps:
• hexulose-6-phosphate synthase,
• ii) Phi:
• hexulose-6-phosphate isomerase,
• iv) AspC:
• aspartate transaminase,
• v) RpiA:
• ribose-5-phosphate isomerase,
• vi) YjhH:
• 2-keto-3-deoxy-galactonate aldolase (which also performs the 4-hydroxy-2-oxobutyrate aldolase reaction),
• vii) Ppc:
• phosphoenolpyruvate carboxylase,
• viii) Asd:
• aspartate-semialdehyde dehydrogenase, and
• ix) ThrA_S345F:
• fused aspartate kinase/homoserine dehydrogenase, wherein the system incorporates the carbon dioxide or bicarbonate into fructose-6-phosphate without rubisco.