Fermentation methods using acetogenic carboxydotrophic bacteria
AssigneeSynata Bio, Inc.
InventorsDavid KEATING, Jennine B. TERRILL
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Abstract
Disclosed are methods for producing from a gaseous substrate comprising CO, CO2, and optionally H2 at least one oxygenated product. Also provided are methods of using a broth deficient in thiamine or thiazole-containing thiamine precursors as a screening tool for suitable ethanol producing acetogenic carboxydotrophic bacteria strains, operating a syngas fermentation without the addition of thiamine or thiazole-containing thiamine precursors, controlling bacterial contamination in a bioreactor, and methods for preparing animal feed and fertilizer.
Claims16
A method of producing from a gaseous substrate comprising CO, CO2, and optionally H2 at least one oxygenated product, the method comprising:
- (a) providing to a bioreactor:
- (1) a gaseous substrate comprising CO, CO2, and optionally H2, (2) at least one acetogenic carboxydotrophic bacterium, and (3) a liquid nutrient medium, and
- (b) providing conditions within the bioreactor for the at least one acetogenic carboxydotrophic bacterium to convert the gaseous substrate to at least one oxygenated product,
- wherein the at least one acetogenic carboxydotrophic bacterium comprises at least one gene selected from thiH, thiS, thiF, and thiG.
A method for producing from a gaseous substrate comprising CO, CO2, and optionally H2 at least one oxygenated product, the method comprising:
- (a) providing to a bioreactor:
- (1) a gaseous substrate comprising CO, CO2, and optionally H2, (2) at least one acetogenic carboxydotrophic bacterium, and (3) a liquid nutrient medium, and
- (b) providing conditions within the bioreactor for the at least one acetogenic carboxydotrophic bacterium to convert the gaseous substrate to at least one oxygenated product,
- wherein the at least one acetogenic carboxydotrophic bacterium does not require exogenously supplied thiamine or thiazole-containing thiamine precursors.
A method of operating a syngas fermentation without the addition of thiamine or thiazole-containing thiamine precursors, the method comprising:
- (a) providing to a bioreactor:
- (1) a gaseous substrate comprising CO, CO2, and optionally H2, (2) at least one acetogenic carboxydotrophic bacterium, and (3) a liquid nutrient medium that does not contain a thiamine or thiazole-containing thiamine precursors;
- and
- (b) providing conditions within the bioreactor for the at least one acetogenic carboxydotrophic bacterium to convert the gaseous substrate to at least one oxygenated product,
- wherein the conditions within the bioreactor create a bioreactor broth, and thiamine or thiazole-containing thiamine precursors is not added to (i) the liquid nutrient medium or (ii) the bioreactor broth before or during syngas fermentation.
A method for producing from a gaseous substrate comprising CO, CO2, and optionally H2 at least one oxygenated product, the method comprising:
- (a) providing to a bioreactor:
- (1) a gaseous substrate comprising CO, CO2, and optionally H2, (2) at least one acetogenic carboxydotrophic bacterium, and (3) a liquid nutrient medium, and
- (b) providing conditions within the bioreactor for the at least one acetogenic carboxydotrophic bacterium to convert the gaseous substrate to at least one oxygenated product,
- wherein the at least one acetogenic carboxydotrophic bacterium comprises at least one exogenous gene selected from thiH, thiS, thiF, and thiG.
Dependencies are inferred from the claim text.