US 2025/0084398 A1 · SYSTEMS FOR MODULATING DRUG METABOLISM BY CELLS, METHODS AND COMPOSITIONS THEREOF

Abstract

The present disclosure provides systems for modulating drug metabolism, specifically in the gut microbiome, by modifying target cell/s and/or population of cells comprising the target cell/s. The disclosed systems comprise the following components: (a) at least one drug metabolism modulating component comprising at least one nucleic acid sequence encoding and/or modulating at least one element participating in metabolism of at least one drug, at least one cas gene and at least one Clustered. Regularly Interspaced Short Palindromic Repeat (CRISPR) array; and (b) at least one selective component comprising at least one protospacer. The at least one protospacer is targeted by at least one spacer of the CRISPR array of (a), thereby inactivating said selective component. The present disclosure further provides, compositions and methods using the disclosed systems and any components thereof in modulation of drug metabolism and in therapeutic applications.

Claims21

Dependent claims

1-60. (canceled)

A system for modulating drug metabolism by modifying target cell/s and/or population of cells comprising said target cell/s, comprising:

(a) at least one drug metabolism modulating component comprising at least one nucleic acid sequence encoding and/or modulating at least one element participating in metabolism of at least one drug, at least one cas gene and at least one Clustered, Regularly Interspaced Short Palindromic Repeat (CRISPR) array;
and
(b) at least one selective component comprising at least one protospacer, wherein said at least one protospacer is targeted by at least one spacer of the CRISPR array of (a), thereby inactivating said selective component.

A method for modulating drug metabolism of a target cell/s and/or population of cells comprising said target cell/s, the method comprising at least one of:

(a) contacting said cell/s or any cell population comprising said cell/s with at least one drug metabolism modulating component comprising at least one nucleic acid sequence encoding or modulating at least one element participating in metabolism of at least one drug, at least one cas gene and at least one CRISPR array;
and
(b) contacting said cells or any cell population comprising said cell/s with at least one selective component comprising at least one protospacer, wherein said at least one protospacer is targeted by at least one spacer of the CRISPR array of (a) to inactivate said selective component;
thereby modulating drug metabolism of said bacterial cell and/or obtaining a population of bacterial cells exhibiting modified metabolism of at least one drug.

1 more element

At least one cell and/or a population of said cells, or any composition or product thereof, exhibiting modified metabolism of at least one drug, wherein said cell and/or cell population is prepared by a method comprising at least one of:

(a) contacting said cell/s or any cell population comprising said cell/s with at least one drug metabolism modulating component comprising at least one nucleic acid sequence encoding or modulating at least one element participating in metabolism of at least one drug, at least one cas gene and at least one CRISPR array;
and
(b) contacting said cells or any cell population comprising said cell/s with at least one selective component comprising at least one protospacer, wherein said at least one protospacer is targeted by at least one spacer of the CRISPR array of (a), to inactivate said selective component.

Dependencies are inferred from the claim text.

Facts

Application

18/294,978Filed 1 Aug 2022

Priority

2 Aug 2021

Published

13 Mar 2025

Term ends

Not estimatedThe source records don’t support an estimate.

Classification22 codes

C12N 15/102
Processes for the isolation, preparation or purification of DNA or RNA: mutagenizing nucleic acids
C12N 15/1037
Isolating an individual clone by screening libraries: screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli…
A61K 31/519
Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim: ortho- or peri-condensed with heterocyclic rings
C07K 14/245
From Enterobacteriaceae , e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia: escherichia

Systems for modulating drug metabolism by cells, methods and compositions thereof

Life of this patent

Systems for modulating drug metabolism by cells, methods and compositions thereof

Life of this patent

Abstract

Claims21