Large scale adeno-associated virus production systems

-3. (canceled)

A method of producing recombinant adeno-associated virus (rAAV), the method comprising the steps of:

• infecting cells with recombinant baculovirus (rBV) at a multiplicity of infection (MOI) of less than 0.01, wherein the rBV has nucleotide sequences for generating rAAV;
• and
• culturing the infected cells to generate rAAV.

-26. (canceled)

-41. (canceled)

A composition comprising recombinant adeno-associated virus (rAAV), wherein the rAAV has at least one of:

• a concentration of encapsidated baculoviral nucleotide sequences that is less than 1E-9 nanograms per nanogram of encapsidated rAAV vector genome.
• a concentration of encapsidated baculoviral nucleotide sequences that encodes at least a portion of a baculoviral DNA polymerase that is less than 1E-2 copies per copy of encapsidated rAAV vector genome;
• a concentration of encapsidated cellular 18S ribosomal RNA gene nucleotide sequences that is less than 1E-3 copies per copy of encapsidated rAAV vector genome.