# US 2025/0084378 A1 · Nonhuman stem cells and their use for production of cultured meat

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- Number: US 2025/0084378 A1
- Document ID: US20250084378A1
- Status: Published application
- Kind: A1
- Application number: 18/723,927
- Filed: 2022-12-20
- Published: 2025-03-13
- Earliest claimed: 2021-12-30
- Assignees: Atelier Meats Corp.
- Applicants: Atelier Meats Corp.
- Inventors: Maghsoud DARIANI; Mohit BHATIA
- Web page: https://patentgrep.com/patent/US20250084378A1

## CPC

- A23L 13/00: Meat products; Meat meal; Preparation or treatment thereof
- C12N 5/0653: {Adipocytes; Adipose tissue}
- C12N 5/0605: {Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly}
- C12N 2506/02: from embryonic cells
- C12N 2509/00: Methods for the dissociation of cells, e.g. specific use of enzymes
- C12N 2533/90: Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Methods for production of nonhuman mammalian cultured meat fit for human consumption involving co-culturing cells isolated from nonhuman mammalian umbilical cord and/or placenta and/or components thereof with extracellular matrix (ECM) derived from nonhuman mammalian placentas and products produced by these methods are provided.

## Claims

### Claim 1

A method for production of nonhuman mammalian cultured meat fit for human consumption, said method comprising co-culturing cells isolated from nonhuman mammalian umbilical cord and/or placenta and/or components thereof with extracellular matrix (ECM) derived from nonhuman mammalian placentas.

### Claim 2 (depends on 1)

The method of claim 1 wherein said cells are derived from stem cells isolated from nonhuman mammalian placenta, umbilical cord, Wharton's Jelly, placental decidua and/or vasculature thereof.

### Claim 3 (depends on 2)

The method of claim 2 wherein said stem cells possess at least one surface marker selected from CD10+, CD13+, CD29+, CD44+, CD73+, CD90+, CD105+ and CD200+.

### Claim 4 (depends on 3)

The method of claim 3 wherein the phenotype of said stem cells is maintained through 10 or more doublings or expansions.

### Claim 5 (depends on 2)

The method of claim 2 wherein the stem cells are expanded and frozen as a master cell bank prior to co-culturing.

### Claim 6 (depends on 2)

The method of claim 2 wherein the stem cells are differentiated into cells of one or more germ layers selected from ectoderm, endoderm and mesoderm.

### Claim 7 (depends on 2)

The method of claim 2 wherein the stem cells are differentiated into adipocytes, chondrocytes, endothelial cells and muscle precursors.

### Claim 8 (depends on 6)

The method of claim 6 wherein different cell types are co-cultured together.

### Claim 9 (depends on 6)

The method of claim 6 wherein the different cell types are cultured with the ECM individually in a specific order.

### Claim 10 (depends on 6)

The method of claim 6 wherein the ECM tissue is cultured with different amounts of cells to affect fat, protein content and/or texture of the cultured meat.

### Claim 11 (depends on 1)

The method of claim 1 wherein the cells are isolated from a nonhuman mammal selected from bovine, porcine, goat or sheep.

### Claim 12 (depends on 1)

A nonhuman mammalian cultured meat fit for consumption produced in accordance with claim 1.

### Claim 13 (depends on 7)

The method of claim 7 wherein different cell types are co-cultured together.

### Claim 14 (depends on 7)

The method of claim 7 wherein the different cell types are cultured with the ECM individually in a specific order.

### Claim 15 (depends on 7)

The method of claim 7 wherein the ECM tissue is cultured with different amounts of cells to affect fat, protein content and/or texture of the cultured meat.