# US 2025/0083204 A1 · Cupriavidus metallidurans cml2, its preparation, and its bioremediation method for heavy metal-contaminated environment

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- Number: US 2025/0083204 A1
- Document ID: US20250083204A1
- Status: Published application
- Kind: A1
- Application number: 18/588,041
- Filed: 2024-02-27
- Published: 2025-03-13
- Earliest claimed: 2023-09-08
- Assignees: Hubei University
- Applicants: Hubei University
- Inventors: Xuejing YU; Yong YANG; Yuan ZHANG; Xianhua ZHANG; Yadong LI; Shan WU; Linjie LI; Chang GAO; Yue LU; Tong WU
- Web page: https://patentgrep.com/patent/US20250083204A1

## CPC

- B09C 1/10: microbiologically, {biologically} or by using enzymes
- B09C 1/105: {using fungi or plants}
- C02F 3/34: characterised by the microorganisms used
- C12N 1/145: Fungi isolates
- C12N 1/205: Bacterial isolates
- C02F 2101/20: Heavy metals or heavy metal compounds
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- C12R 2001/645: Fungi {; Processes using fungi}
- B09C: RECLAMATION OF CONTAMINATED SOIL (gatherers for removing stone or the like from the soil A01B43/00; sterilising soil by steam A01G11/00; removing undesirable matter, e.g. rubbish, from the land E01H15/00)
- C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

Embodiments of the present disclosure disclose a Cupriavidus metallidurans CML2, wherein the Cupriavidus metallidurans CML2 is deposited in the China Center for Type Culture Collection with a depository number CCTCC NO: M20231365, and a 16s rDNA of the Cupriavidus metallidurans CML2 has a nucleotide sequence of SEQ ID No. 1.

## Claims

### Claim at position 1

1-13. (canceled)

### Claim 14

A bioremediation method for a cadmium-contaminated environment comprising:
adding a bacterial inoculum comprising Cupriavidus metallidurans CML2 to the cadmium-contaminated environment; and
incubating the bacterial inoculum in the cadmium-contaminated environment for 1 hour to 1 week to reduce cadmium from the cadmium-contaminated environment; wherein
the Cupriavidus metallidurans CML2 is deposited in the China Center for Type Culture Collection with a depository number CCTCC NO: M20231365 on Jul. 24, 2023, and a 16s rDNA of the Cupriavidus metallidurans CML2 has a nucleotide sequence of SEQ ID No. 1.

### Claim 15 (depends on 14)

The bioremediation method according to claim 14, wherein the reducing cadmium from the cadmium-contaminated environment is to reduce cadmium content in cadmium-containing soil or wastewater.

### Claim 16 (depends on 14)

The bioremediation method according to claim 14, wherein the reducing cadmium from the cadmium-contaminated environment is to reduce a stress on rice growth caused by cadmium ions or to reduce the cadmium content in rice.