# US 2025/0083140 A1 · Methods of isolating biomarker cells

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- Number: US 2025/0083140 A1
- Document ID: US20250083140A1
- Status: Published application
- Kind: A1
- Application number: 18/631,612
- Filed: 2024-04-10
- Published: 2025-03-13
- Earliest claimed: 2017-04-20
- Assignees: BioFluidica, Inc.
- Applicants: Biofluidica, Inc.
- Inventors: Rolf Muller; Scott Beach; Judy Muller-Cohn
- Web page: https://patentgrep.com/patent/US20250083140A1

## CPC

- B01L 3/0237: {Details of electronic control, e.g. relating to user interface}
- B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
- G01N 35/1016: {Control of the volume dispensed or introduced}
- B01L 3/502753: {characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation}
- B01L 2200/0652: Sorting or classification of particles or molecules
- B01L 2200/0668: Trapping microscopic beads
- B01L 2200/0689: Sealing
- B01L 2200/146: Employing pressure sensors
- B01L 2300/0663: Whole sensors
- G01N 35/1011: {Control of the position or alignment of the transfer device}
- G01N 35/109: {with two horizontal degrees of freedom}
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The presently disclosed subject matter provides methods of isolating biomarker cells from a liquid sample, and related methods of capturing DNA or RNA released from biomarker cells.

## Claims

### Claim 1

A method of isolating biomarker cells from a liquid sample comprising:
a) providing a fluid-tight flow system comprising:
a microfluidic chip comprising an inlet port in fluid communication with an outlet port and a cell selection module, wherein said cell selection module comprises a capture bed in fluid communication with the inlet port and the outlet port, and wherein the capture bed comprises a plurality of isolation channels configured to isolate biomarker cells from the liquid sample or solid supports configured to bind to biomarker cells;
a first automated pipetting channel comprising a first pump, and a first pipette tip containing a liquid sample and coupled to the inlet port;
a second automated pipetting channel comprising a second pump, and a second pipette tip coupled to the outlet port; and
a non-transitory computer readable medium in communication with the first pump and the second pump, and programmed to command the first pump of the first automated pipetting channel and the second pump of the second automated pipetting channel to control flow of the liquid sample through the microfluidic chip, wherein the non-transitory computer readable medium comprises programming to control flow of the liquid sample through the cell selection module;
b) controlling flow of the liquid sample through the cell selection module; and
c) isolating biomarker cells from the liquid sample.

### Claim 2 (depends on 1)

The method of claim 1, wherein the biomarker cells are circulating tumor cells or circulating leukemic cells.

### Claim 3 (depends on 1)

The method of claim 1, wherein the method further comprises lysing biomarker cells; and capturing DNA or RNA released from lysed biomarker cells.

### Claim 4 (depends on 1)

The method of claim 1, wherein the liquid sample is blood, saliva, lymphatic fluid, cells suspended in fluid, synovial fluid, semen, urine, cerebrospinal fluid, or amniotic fluid.