Bacteriophage lambda-vaccine system

Abstract

Bacteriophage λ are disclosed herein that include a head, a tail, and a lambda genome comprising a nucleic acid sequence encoding a fusion protein comprising a D protein linked to heterologous antigen, wherein the nucleic acid sequence is inserted into a native gene D locus adjacent to gene E, in the lambda genome, and wherein expression of the fusion protein results in the head of the bacteriophage λ comprising the fusion protein. Host bacterial cells also disclosed herein that are infected with the bacteriophage λ. In addition, immunogenic compositions are disclosed that include an effective amount of the bacteriophage λ. Methods also are disclosed for inducing an immune response to the heterologous antigen in a subject. Furthermore, methods are disclosed for preparing these bacteriophage λ.

Dependencies are inferred from the claim text.