# US 2025/0081986 A1 · Canola Protein Concentrate and Process for Production

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- Number: US 2025/0081986 A1
- Document ID: US20250081986A1
- Status: Published application
- Kind: A1
- Application number: 18/792,399
- Filed: 2024-08-01
- Published: 2025-03-13
- Earliest claimed: 2023-08-11
- Applicants: Louis Dreyfus Company Plant Proteins LLC
- Inventors: Prasanth Kumar Sasidharan Pillai; Manoj Kumar
- Web page: https://patentgrep.com/patent/US20250081986A1

## CPC

- A23J 1/144: {Desolventization}
- A23L 25/30: {Mashed or comminuted products, e.g. pulp, pastes, meal, powders; Products made therefrom, e.g. blocks, flakes, snacks; Liquid or semi-liquid products (A23L25/10 and A23L25/40 take precedence)}
- A23L 27/60: Salad dressings; Mayonnaise; Ketchup
- A23L 33/185: Vegetable proteins
- A23J: PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)

## Abstract

Provided herein are a processes for producing a canola protein concentrate (CPC) from de-oiled canola flour that is economical and produces canola protein concentrate that has unique composition and functionality.

## Claims

### Claim 1

A canola protein concentrate process comprising:
a. extracting, in a Nutsche filter, in 30-70% ethanol in water (by volume) de-oiled, desolvated canola seed flour, said canola seed flour having ≤3% oil, the extraction comprising the parameters of:
i) a maintained temperature of 30-62° C.,
ii) a pH range of pH 4-6,
b. removing the ethanol from the canola seed protein-concentrate and water,
c. optionally washing the canola protein concentrate with water at pH 4-5, then diluting the sunflower protein concentrate with water at pH 7 to create a slurry, then homogenizing said slurry,
d. optionally sterilizing and/or pasteurizing the wet canola protein concentrate, and
e. drying the wet canola flour protein concentrate to a moisture range of 4-10%.

### Claim 2

(canceled)

### Claim 3 (depends on 1)

The process of claim 1, wherein the de-oiled, desolvated canola seed flour is a fine powder fraction (hulls separated and removed) of de-oiled, desolvated canola seed flour.

### Claim 4 (depends on 3)

The process of claim 3, wherein the fine powder fraction consists essentially of particles of ≤500 μm.

### Claim 5 (depends on 3)

The process of claim 3, wherein the fine powder fraction is obtained by sieving.

### Claim 6 (depends on 3)

The process of claim 3, wherein the fine powder fraction is obtained by air classification.

### Claim 7 (depends on 1)

The process of claim 1, wherein the protein content of the de-oiled, desolvated canola seed is 45-70 wt % protein (N×6.25) on a dry basis.

### Claim 8 (depends on 1)

The process of claim 1, wherein the protein content of the de-oiled, desolvated canola seed is 48-53 wt % protein (N×6.25) on a dry basis.

### Claim 9 (depends on 1)

The process of claim 1, wherein the de-oiled, desolvated canola seed flour contains <1% oil.

### Claim 10 (depends on 1)

The process of claim 1, wherein steps a. is carried out at 40-60° C.

### Claim 11 (depends on 1)

The process of claim 1, wherein step a. is carried out at pH 4-5.

### Claim 12 (depends on 1)

The process of claim 1, wherein step a. is carried out for 15-30 minutes.

### Claim 13 (depends on 1)

The process of claim 1, wherein step e. is performed using spray dryer, ring dryer, dispersion dryer, drum dryer or fluid bed dryer.

### Claim 14 (depends on 1)

The process of claim 1, wherein steps b., c. and e. are carried out at about ≤60° C.

### Claim 15 (depends on 1)

A canola protein concentrate produced by the process of claim 1.

### Claim 16 (depends on 15)

The canola protein concentrate of claim 15 having a protein content of 55-73% protein by weight (N×6.25) on a dry basis.

### Claim 17 (depends on 15)

The canola protein concentrate of claim 15, wherein the protein component of the canola protein concentrate comprises 5-20% oleosin.

### Claim 18 (depends on 15)

The canola protein concentrate of claim 15 wherein the protein component of the canola protein concentrate comprises:
a. 50-65% cruciferin,
b. 5-20% oleosin, and
c. 8-15% napin.

### Claim 19 (depends on 18)

The canola protein concentrate of claim 18, further comprising:
d. 1-3% oil,
e. ≤4% phytates,
f. ≤40% carbohydrates,
g. ≤1% polyphenol,
h. ≤0.5% glucosinolates, and
i. ≤5% ash.

### Claim 20 (depends on 15)

The canola protein concentrate of claim 15, wherein the protein is substantially in native form.

### Claim 21

A canola protein concentrate, wherein the protein component of the canola protein concentrate comprises 5-20% oleosin.

### Claim 22 (depends on 21)

The canola protein concentrate of claim 21, wherein the protein component of the canola protein concentrate comprising 12-15% oleosin.

### Claim 23 (depends on 21)

The canola protein concentrate of claim 21, wherein the protein component of the canola protein concentrate comprises:
a. 50-65% cruciferin,
b. 5-20% oleosin, and
c. 8-15% napin.

### Claim 24 (depends on 21)

The canola protein concentrate of claim 21, comprising 55-73% protein by weight (N×6.25) on a dry basis.

### Claim 25 (depends on 21)

The canola protein concentrate of claim 21, wherein the protein is substantially in native form.

### Claim 26 (depends on 15, 21)

The canola protein concentrate of claim 15 or 21 having a foaming expansion capacity of 180-300% at 4-5% (w/w) canola protein concentrate in water.

### Claim 27 (depends on 15, 21)

The canola protein concentrate of claim 15 or 21 having a foaming stability of 50-80% at 4-5% (w/w) canola protein concentrate in water after 1 hour.

### Claim 28 (depends on 15, 21)

The canola protein concentrate of claim 15 or 21 having an emulsion stability of 100% at ≥4% (w/w) canola protein concentrate in oil/water (50/50) after 5 days cold storage.

### Claim 29 (depends on 28)

The canola protein concentrate of claim 28, wherein the emulsion is prepared at pH 5-7.

### Claim 30 (depends on 15, 21)

The canola protein concentrate of claim 15 or 21 having a least gelation concentration of 5-8%.

### Claim 31 (depends on 15, 21)

The canola protein concentrate of claim 15 or 21 having a water holding capacity of 4-10 grams per gram (g/g).

### Claim 32 (depends on 15, 21)

The canola protein concentrate of claim 15 or 21 having an oil holding capacity of 3-9 grams per gram (g/g).

### Claim 33 (depends on 15, 21)

Use of the canola protein concentrate of claim 15 or 21 in the production of a foam.

### Claim 34 (depends on 15, 21)

Use of the canola protein concentrate of claim 15 or 21 in the production of a gel.

### Claim 35 (depends on 15, 21)

Use of the canola protein concentrate of claim 15 or 21 in the production of an emulsion.

### Claim 36 (depends on 35)

The use of claim 35, wherein the emulsion is an oil/water emulsion.

### Claim 37 (depends on 15, 21)

A foam comprising the canola protein concentrate of claim 15 or 21.

### Claim 38 (depends on 15, 21)

A gel comprising the canola protein concentrate of claim 15 or 21.

### Claim 39 (depends on 15, 21)

An emulsion comprising the canola protein concentrate of claim 15 or 21.

### Claim 40 (depends on 15, 21)

Use of the canola protein concentrate of claim 15 or 21 in a food or beverage application.

### Claim 41 (depends on 40)

The use of claim 40, wherein the food or beverage application is selected from milk shake, protein bars, meat analogues, confectionary, condiments, mayonnaise, salad dressing, nutritional supplements and diary alternatives.

### Claim 42 (depends on 41)

The use of claim 41, wherein the dairy alternative is selected from creamers, ice cream, yogurt, buttermilk and cheese.

### Claim 43 (depends on 15, 21)

A food or beverage comprising the canola seed protein concentrate of claim 15 or 21.