# US 2025/0081908 A1 · Microalgae-based soil inoculating system and methods of use > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2025/0081908 A1 - Document ID: US20250081908A1 - Status: Published application - Kind: A1 - Application number: 18/889,198 - Filed: 2024-09-18 - Published: 2025-03-13 - Earliest claimed: 2011-05-03 - Assignees: Myland Limited - Applicants: Myland Company, Inc - Inventors: Andrew D. Ayers; Mark R. Edwards; Dane Hague; Robert Thompson - Web page: https://patentgrep.com/patent/US20250081908A1 ## CPC - A01G 33/00: Cultivation of seaweed {or algae} - C05D 9/02: containing trace elements - C05F 11/08: Organic fertilisers containing added bacterial cultures, mycelia or the like - C05G 5/20: Liquid fertilisers (layered or coated C05G5/30; incorporated into a matrix C05G5/40) - C12M 21/02: {Photobioreactors (culturing algae A01G33/00, A01H4/001, C12N1/12)} - C12M 23/52: {Mobile; Means for transporting the apparatus (transportable laboratories B01L1/52)} - C12M 29/04: {Filters; Permeable or porous membranes or plates, e.g. dialysis} - C12M 37/00: {Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination (C12M23/38 takes precedence; filtration in general and filters per se B01D24/00-B01D41/00; autoclaves B01J3/04; treatment of microorganisms with electrical or wave energy C12N13/00)} - C12M 43/00: {Combinations of bioreactors or fermenters with other apparatus} - C12N 1/12: Unicellular algae; Culture media therefor (as new plants A01H13/00) - Y02W 10/37: using solar energy - A01G: HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING (picking of fruits, vegetables, hops or the like A01D46/00; propagating unicellular algae C12N1/12) - C05D: INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE - C05F: ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE - C05G: MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS (organic fertilisers containing added bacterial cultures, mycelia, or the like C05F11/08; organic fertilisers containing plant vitamins or hormones C05F11/10); FERTILISERS CHARACTERISED BY THEIR FORM - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - Y02W: CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT ## Abstract Some embodiments include a microalgae culturing system including a bioreactor adapted to propagate microalgae in a culture solution using in combination at least one of natural and artificial light, and at least one nutrient including at least a carbon source, where the microalgae are freely suspended in and form part of the culture solution. A microalgae feed source is coupled to the bioreactor and a first controller between a water conditioning assembly and the bioreactor. The water conditioning assembly is coupled as an input of supply water to the bioreactor, and configured to condition the supply water to a specified purity that enables substantially unhindered growth of the microalgae in the culture solution to a specified concentration, and the first controller is configured to control supply of the microalgae feed source to the bioreactor. ## Claims ### Claim at position 1 1-34. (canceled) ### Claim 35 A system for soil enrichment comprising: one or more bioreactors, an ozone generator, a nutrient feed supply, and a carbon dioxide source; wherein the carbon dioxide source is configured to supply carbon dioxide to a lower part of each of the one or more bioreactors; wherein the system is configured to receive water from an on-site water source of a farm; wherein the system is configured to provide live microalgae cells to an effluent of the one or more bioreactors; and wherein the effluent is configured to feed the live microalgae cells to a water flow of an irrigation system. ### Claim 36 (depends on 35) The system of claim 35, further comprising one or more monitoring devices. ### Claim 37 (depends on 36) The system of claim 36, wherein the one or more monitoring devices are configured to measure a microalgae titer in the effluent. ### Claim 38 (depends on 36) The system of claim 36, wherein the one or more monitoring devices are configured to measure nutrient content in the effluent. ### Claim 39 (depends on 35) The system of claim 35, wherein the system is configured to support a flow-through capacity of about 0.35-0.7 gallons per minute. ### Claim 40 (depends on 39) The system of claim 39, wherein the system is configured to support a field in a range of 200-1000 acres. ### Claim 41 (depends on 35) The system of claim 35, further comprising a flow-imaging device configured to generate images of the live microalgae cells. ### Claim 42 (depends on 35) The system of claim 35, wherein the system is placed within an enclosure. ### Claim 43 (depends on 35) The system of claim 35, wherein the one or more bioreactors comprise light-permeable walls. ### Claim 44 (depends on 36) The system of claim 36, wherein the system includes eight bioreactors. ### Claim 45 (depends on 36) The system of claim 36, further including the ozone generator. ### Claim 46 (depends on 45) The system of claim 45, further including a pressurized air supply. ### Claim 47 (depends on 46) The system of claim 46, further including an ultra-violet light system. ### Claim 48 (depends on 47) The system of claim 47, further comprising a water tank; and where in the water tank is configured to receive the water from the on-site water source of the farm. ### Claim 49 (depends on 48) The system of claim 48, wherein the system is configured to provide about 800,000 live microalgae cells per second at a water flowrate of about 0.35 gallons per minute to the effluent of the one or more bioreactors. ### Claim 50 (depends on 49) The system of claim 49, further comprising a mechanical agitator. ### Claim 51 A system for soil enrichment comprising: a trailer, one or more bioreactors, an ozone generator, a nutrient feed supply, and a carbon dioxide source; wherein the one or more bioreactors, the ozone generator, the nutrient feed supply, and the carbon dioxide source are each mounted on the trailer; wherein the carbon dioxide source is configured to supply carbon dioxide to a lower part of each of the one or more bioreactors; wherein the system is configured to receive water from an on-site water source of a farm; wherein the system is configured to provide live microalgae cells to an effluent of the one or more bioreactors; and wherein the effluent is configured to feed the live microalgae cells to a water flow of an irrigation system. ### Claim 52 (depends on 51) The system of claim 51, wherein the system is configured to support a field in a range of 200-1000 acres. ### Claim 53 (depends on 52) The system of claim 52, wherein the system is configured to support a flow-through capacity of about 0.35-0.7 gallons per minute. ### Claim 54 (depends on 53) The system of claim 53, wherein the system is configured to provide about 800,000 live microalgae cells per second at a water flowrate of about 0.35 gallons per minute to the effluent of the one or more bioreactors. ### Claim 55 A system for soil enrichment comprising: a trailer, one or more bioreactors, an ozone generator, a nutrient feed supply, a carbon dioxide source, and one or more monitoring devices; wherein the one or more bioreactors, the ozone generator, the nutrient feed supply, and the carbon dioxide source are each mounted on the trailer; wherein the carbon dioxide source is configured to supply carbon dioxide to a lower part of each of the one or more bioreactors; wherein the system is configured to receive water from an on-site water source of a farm; wherein the system is configured to support a flow-through capacity of about 0.35-0.7 gallons per minute; wherein the system is configured to provide about 800,000 live microalgae cells per second at a water flowrate of about 0.35 gallons per minute to an effluent of the one or more bioreactors; and wherein the effluent is configured to feed the live microalgae cells to a water flow of an irrigation system.