Biosynthesis of oxygenated hydrocarbons
AssigneeGinkgo Bioworks, Inc.
InventorsElena Brevnova, Bernardo Moura Torres da Costa, Krupanandan Haranahalli, Gabriel Rodriguez, Michael Torrens-Spence, Justin Vento
Life of this patent
Estimating the term…
Abstract
Described herein are CB5 polypeptides for the biosynthesis of oxygenated hydrocarbons in modified host cells.
Claims34
-10. (canceled)
A host cell that comprises a heterologous polynucleotide encoding a cytochrome b5 (CB5), wherein the host cell is capable of producing more of an oxygenated hydrocarbon than a control host cell that does not comprise the heterologous polynucleotide, and wherein the CB5 comprises:
- a) the amino acid sequence X1X2X3X4X5X6X7EX8IX9X10YTGLSPX11X12FFTX13LAX14X15X16X17VX18X19X20X21SX22X23FX24X25X26X27X28X29X30X31 (SEQ ID NO:
- 50), wherein:
- (i) X1 is the amino acid E or Q;
- (ii) X2 is the amino acid L or V;
- (iii) X3 is the amino acid Y or W;
- (iv) X4 is the amino acid W or E;
- (v) X5 is the amino acid K or T;
- (vi) X6 is the amino acid A or L;
- (vii) X7 is the amino acid M or K;
- (viii) X8 is the amino acid Q or A;
- (ix) X9 is the amino acid A or V;
- (x) X10 is the amino acid W or A;
- (xi) X11 is the amino acid T or A;
- (xii) X12 is the amino acid A or T;
- (xiii) X13 is the amino acid I or V;
- (xiv) X14 is the amino acid S or L;
- (xv) X15 is the amino acid M or G;
- (xvi) X16 is the amino acid I or L;
- (xvii) X17 is the amino acid F or A;
- (xviii) X15 is the amino acid F or Y;
- (xix) X19 is the amino acid Q or Y;
- (xx) X20 is the amino acid M or V;
- (xxi) X21 is the amino acid V or I;
- (xxii) X22 is the amino acid S or G;
- (xxiii) X23 is the amino acid M or F;
- (xxiv) X24 is the amino acid V or G;
- (xxv) X25 is the amino acid S or T;
- (xxvi) X26 is the amino acid P or S;
- (xxvii) X27 is the amino acid E or D;
- (xxviii) X28 is the amino acid E or Y;
- (xxix) X29 is the amino acid F or G;
- (xxx) X30 is the amino acid N or S;
- and/or
- (xxxi) X31 is the amino acid K or H;
- b) the amino acid sequence X1VQX2GX3X4X5EX6X7LX8X9YDGSDX10X11KPLLMAIKGQIYDVSX12X13RMF (SEQ ID NO:
- 51), wherein:
- (i) X1 is the amino acid P or A;
- (ii) X2 is the amino acid V or I;
- (iii) X3 is the amino acid E or Q;
- (iv) X4 is the amino acid I or L;
- (v) X5 is the amino acid S or T;
- (vi) X6 is the amino acid E or Q;
- (vii) X7 is the amino acid E or Q;
- (viii) X8 is the amino acid K or R;
- (ix) X9 is the amino acid Q or A;
- (x) X10 is the amino acid S or P;
- (xi) X11 is the amino acid K or N;
- (xii) X12 is the amino acid Q or S;
- and/or
- (xiii) X13 is the amino acid S or G;
- c) the amino acid sequence LAX1X2SFX3X4X5DX6TGX7IX8GLX9X10X11ELX12X13LQDWEYKFMX14KYVKVGX15X1 6 (SEQ ID NO:
- 52), wherein:
- (i) X1 is the amino acid K or L;
- (ii) X2 is the amino acid M or L;
- (iii) X3 is the amino acid E or K;
- (iv) X4 is the amino acid E or P;
- (v) X5 is the amino acid K or E;
- (vi) X6 is the amino acid L or I;
- (vii) X7 is the amino acid D or N;
- (viii) X8 is the amino acid S or E;
- (ix) X9 is the amino acid G or S;
- (x) X10 is the amino acid P or E;
- (xi) X11 is the amino acid F or E;
- (xii) X12 is the amino acid E or V;
- (xiii) X13 is the amino acid A or I;
- (xiv) X14 is the amino acid S or E;
- (xv) X15 is the amino acid T or E;
- and/or
- (xvi) X16 is the amino acid V or L;
- d) the amino acid sequence X1X2X3EX4GX5X6X7X8X9X10D (SEQ ID NO:
- 53), wherein:
- (i) X1 is the amino acid K or E;
- (ii) X2 is the amino acid P or H;
- (iii) X3 is the amino acid A or S;
- (iv) X4 is the amino acid D or N;
- (v) X5 is the amino acid P or H;
- (vi) X6 is the amino acid S or R;
- (vii) X7 is the amino acid E or N;
- (viii) X8 is the amino acid S or F;
- (ix) X9 is the amino acid Q or E;
- and/or
- (x) X10 is the amino acid A or I;
- and/or
- e) the amino acid sequence DATX1X2FX3X4X5VGHS (SEQ ID NO:
- 31), wherein:
- (i) X1 is the amino acid E, D, or N;
- (ii) X2 is the amino acid A or D;
- (iii) X3 is the amino acid E or D;
- (iv) X4 is the amino acid D or N;
- and/or
- (v) X5 is the amino acid V or A.
(canceled)
-17. (canceled)
-23. (canceled)
A host cell that comprises a heterologous polynucleotide encoding a cytochrome b5 (CB5), wherein the CB5 comprises a sequence that is at least 90% identical to any one of SEQ ID NOs:
- 1-10, 29, 30, 32, 33, 34, 77, 78, 79, 80, 81, 82, 118, and 318 and wherein the host cell is capable of producing an oxygenated hydrocarbon.
-26. (canceled)
(canceled)
A host cell that comprises a heterologous polynucleotide encoding a cytochrome b5 (CB5), wherein the CB5 comprises:
- a) the amino acid sequence ILRVSFRKYRKAIEQ (SEQ ID NO:
- 54);
- b) the amino acid sequence RAFRPSIRFKKSHSTVPT (SEQ ID NO:
- 55);
- c) the amino acid sequence KNTLYVGG (SEQ ID NO:
- 56);
- and/or
- d) the amino acid sequence DQATQKHRSFGFVTFLEKED (SEQ ID NO:
- 57)
- and wherein the host cell is capable of producing more of an oxygenated hydrocarbon than a control host cell that does not comprise the heterologous polynucleotide.
-34. (canceled)
-43. (canceled)
(canceled)
-56. (canceled)
-62. (canceled)
-71. (canceled)
-74. (canceled)
-78. (canceled)
Dependencies are inferred from the claim text.