Proteins for regulation of symbiotic infection and associated regulatory elements
AssigneeUniversity of Freiburg
InventorsThomas Ott, Guofeng Zhang
Life of this patent
Estimating the term…
Abstract
The present disclosure provides novel DNA molecules and constructs encoding proteins for promoting or increasing symbiotic infection in plants. The disclosure also provides DNA molecules useful for expressing proteins in an infection-specific expression pattern. The instant disclosure also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the disclosed DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.
Claims35
A recombinant DNA molecule comprising a heterologous promoter operably linked to a polynucleotide segment encoding a subtilase protein, homolog, or fragment thereof, wherein:
- a) said protein comprises an amino acid sequence of SEQ ID NO:3;
- b) said protein comprises an amino acid sequence having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or about 100% sequence identity to SEQ ID NO:3;
- or
- c) said polynucleotide segment hybridizes under stringent hybridization conditions to a polynucleotide having the nucleotide sequence of SEQ ID NO:
- 3.
A method for increasing symbiotic nitrogen fixation in a plant, said method comprising:
- a) expressing a subtilase protein or fragment or variant thereof as set forth in SEQ ID NO:3 or at least 70% identical to SEQ ID NO:
- 3 having subtilase activity in a plant;
- and
- b) contacting said plant with an effective amount of one or more rhizobia bacterium, arbuscular mycorrhiza fungi, or a combination thereof.
A recombinant DNA molecule comprising a polynucleotide sequence selected from the group consisting of:
- a) a sequence with at least 70 percent sequence identity to SEQ ID NO:4;
- b) a sequence comprising SEQ ID NO:4;
- c) a fragment of a sequence having at least 85 percent sequence identity to SEQ ID NO:4, wherein the fragment has gene-regulatory activity;
- and
- d) a fragment of SEQ ID NO:4, wherein the fragment has gene-regulatory activity;
- wherein said sequence is operably linked to a heterologous transcribable polynucleotide molecule.
Dependencies are inferred from the claim text.