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US 2025/0075035 A1Published application
Agent for raising melting temperature (tm value) of nucleic acid
AssigneeNitto Boseki Co., Ltd.
InventorsNahohiro FUJIMURA, Ami TACHIBANA, Tomoaki UCHIKI, Minoru TAKEUCHI, Yoko TERUUCHI, Koji WATANABE
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Abstract
Provided is a new agent for increasing the melting temperature (Tm value) of a nucleic acid. The present invention uses a specific (di)allylamine-based compound that meets a specific relationship between the molecular weight of the (di)allylamine-based compound and a numerical value calculated from a specific parameter related to the chemical structure of the (di)allylamine-based compound.
Claims21
An agent for raising a melting temperature (Tm value) of a nucleic acid, comprising
- a (di)allylamine-based (co)polymer, wherein the (di)allylamine-based co(polymer) comprises:
- at least one type of (di)allylamine-based constituent unit selected from the group consisting of
- a constituent unit (A) having a structure represented by General Formula (I-a), or General Formula (I-b), or a structure of an acid addition salt thereof:
- wherein R1 represents an alkyl group having 1 to 10 carbon atoms that may have a hydroxyl group, a cycloalkyl group having 5 to 10 carbon atoms, or an aralkyl group having 7 to 10 carbon atoms, and
- a constituent unit (B) having a structure represented by General Formula (I-d), or General Formula (I-e):
- wherein R2 and R3 each independently represents a hydrogen atom, an alkyl group having 1 to 20 carbon atoms that may have a hydroxyl group, a cycloalkyl group having 5 to 20 carbon atoms, or an aralkyl group having 7 to 20 carbon atoms, at least one of R2 and R3 being not a hydrogen atom, Xa− represents a counter ion, and a represents the valency number of the counter ion;
- and
- is a compound in which a chemical structure parameter P calculated by the following Formula (1) is 2.50 to 4.50, and the chemical structure parameter P and a molecular weight M are in a relationship satisfying the following Formula (2):
- P
- =
- ∑
- i
- =
- 1
- n
- m
- i
- (
- ∑
- j
- =
- 1
- 4
- j
- ×
- a
- ij
- +
- o
- i
- +
- 2
- ×
- s
- i
- )
- ∑
- i
- =
- 1
- n
- m
- i
- (
- 1
- )
- wherein n represents the number o constituent units constituting the (di)allylamine-based compound, mi represents a molar ratio of a constituent unit i, aij represents the number of j-class amines (or ammonium salts) in the constituent unit i, oi represents the number of carbonyl groups in the constituent unit i, and si represents the number of sulfonyl groups in the constituent unit i
- 2.
- 11
- ≦
- P
- 3
- /
- M
- 1
- /
- 4
- ≦
- 6.
- 92.
- (
- 2
- )
60 more elements
A method for raising a melting temperature (Tm value) of a nucleic acid, comprising:
- mixing the nucleic acid with a (di)allylamine-based (co)polymer in a solution, wherein the (di)allylamine-based compound comprises:
- at least one type of (di)allylamine-based constituent unit selected from the group consisting of
- a constituent unit (A) having a structure represented by General Formula (I-a), or General Formula (I-b), or a structure of an acid addition salt thereof:
- wherein R1 represents an alkyl group having 1 to 10 carbon atoms that may have a hydroxyl group, a cycloalkyl group having 5 to 10 carbon atoms, or an aralkyl group having 7 to 10 carbon atoms, and
- a constituent unit (B) having a structure represented by General Formula (I-d), or General Formula (I-e):
- wherein R2 and R3 each independently represents a hydrogen atom, an alkyl group having 1 to 20 carbon atoms that may have a hydroxyl group, a cycloalkyl group having 5 to 20 carbon atoms, or an aralkyl group having 7 to 20 carbon atoms, at least one of R2 and R3 being not a hydrogen atom, Xa− represents a counter ion, and a represents the valency number of the counter ion;
- and
- is a compound in which a chemical structure parameter P calculated by the following Formula (1) is 2.50 to 4.50, and the chemical structure parameter P and a molecular weight M are in a relationship satisfying the following Formula (2):
- P
- =
- ∑
- i
- =
- 1
- n
- m
- i
- (
- ∑
- j
- =
- 1
- 4
- j
- ×
- a
- ij
- +
- o
- i
- +
- 2
- ×
- s
- i
- )
- ∑
- i
- =
- 1
- n
- m
- i
- (
- 1
- )
- wherein n represents the number of constituent units constituting the (di)allylamine-based compound, mi represents a molar ratio of a constituent unit i, aij represents the number of j-class amines (or ammonium salts) in the constituent unit i, oi represents the number of carbonyl groups in the constituent unit i, and si represents the number of sulfonyl groups in the constituent unit i
- 2.
- 11
- ≦
- P
- 3
- /
- M
- 1
- /
- 4
- ≦
- 6.
- 92.
- (
- 2
- )
60 more elements
Dependencies are inferred from the claim text.