Nanoparticles comprising fusion protein of single-chain variable fragment and ferritin, and use thereof

A method for preparing nanoparticles, comprising the steps of:

• (a) preparing an expression vector comprising a polynucleotide encoding a fusion protein, which consists of a ferritin protein;
• a single-chain variable fragment which is bound to an N-terminus of the ferritin protein;
• and an N-terminus domain (hLysRS N-terminal appended RNA interacting domain;
• hRID) which is isolated from a human-derived lysyl tRNA synthetase that is bound to an N-terminus of the single-chain variable fragment;
• (b) preparing a transformant by introducing the expression vector into a host cell;
• (c) culturing the transformant to induce expression of the fusion protein;
• and
• (d) purifying nanoparticles formed by self-assembly of the expressed fusion protein.