Separation matrices and methods for liquid chromatography with improved linear flow velocity
AssigneeThe Purolite Company
InventorSerguei Rudolfovich Kosvintsev
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Abstract
Methods of performing liquid chromatography using a mobile phase and a stationary phase, wherein the stationary phase includes a separation matrix comprising spheroidal polymer beads prepared by dispersing a hydrocolloid or other gel forming compound or a water-soluble polymerizable monomer through a plurality of holes in a membrane under conditions sufficient to form beads with a volume median particle diameter of less than about 300 μm and a SPAN of less than 0.6 to increase linear flow velocity (cm/h) of the mobile phase compared to polymer beads having a SPAN of 0.6 or greater are disclosed. Methods of increasing linear flow velocity of the mobile phase as well as separation matrices are further disclosed.
Claims20
A method of performing liquid chromatography, comprising:
- contacting a mobile phase and a stationary phase within a column, wherein the stationary phase comprises a separation matrix comprising spheroidal polymer beads prepared by dispersing a hydrocolloid or other gel forming compound or a water-soluble polymerizable monomer through a plurality of holes in a membrane under conditions sufficient to form spheroidal polymer beads having a volume median particle diameter up to about 300 μm;
- wherein the mobile phase linear flow velocity (cm/h) is increased when the spheroidal polymer beads have a SPAN of less than about 0.6 compared to spheroidal polymer beads having a SPAN of more than 0.6.
A method of increasing linear flow velocity (cm/h) of a mobile phase moving through a stationary phase in a liquid chromatography process comprising:
- contacting a mobile phase and a stationary phase within a column, wherein the stationary phase comprises a separation matrix comprising spheroidal polymer beads prepared by dispersing a hydrocolloid or other gel forming compound or a water-soluble polymerizable monomer through a plurality of holes in a membrane under conditions sufficient to form spheroidal polymer beads having a volume median particle diameter up to about 300 μm;
- and
- wherein a linear flow velocity (cm/h) of the mobile phase is increased by the use of spheroidal polymer beads having a SPAN of less than about 0.6.
A separation matrix for liquid chromatography, comprising:
- a stationary phase housed within a column,
- wherein the stationary phase comprises a separation matrix comprising spheroidal polymer beads prepared by dispersing a hydrocolloid or other gel forming compound or a water-soluble polymerizable monomer through a plurality of holes in a membrane under conditions sufficient to form spheroidal polymer beads having a volume average particle diameter up to about 300 μm and having a SPAN of less than about 0.6,
- wherein the stationary phase permits a mobile phase to pass through the stationary phase in the column at an increased linear flow velocity (cm/h) compared to a stationary phase comprising spheroidal polymer beads having a SPAN greater than 0.6.
Dependencies are inferred from the claim text.