Temperature-responsive nano-biomaterials from genetically encoded farnesylated proteins
AssigneeSyracuse University
InventorsDavoud Mozhdehi, Shahadat Hossain, James Hougland
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Abstract
A prokaryote was genetically engineered to develop operationally simple, high-yield biosynthetic route for the production of farnesylated proteins. The recombinant organism was modified to express a target protein, a peptide sequence fused to the target protein at a C-terminus, and an alpha and a beta subunit of a prenyltrasferase. The prenyltrasferase may be farnesyltransferase or geranylgeranyl transferase, and the peptide sequence may comprise cysteine, two hydrophobic amino acids, and an amino acid having selectivity to farnesyltransferase or geranylgeranyl transferase.
Claims20
A recombinant organism for forming a lipidated protein, comprising a host organism modified to include a first gene expressing an alpha subunit and a beta subunit of a transferase that will attach a lipid from a lipid donor to a protein and to include a second gene expressing the protein, wherein the protein includes a substrate of the transferase.
A method of producing a lipidated protein, comprising:
- modifying a host organism to include a first gene expressing an alpha subunit and a beta subunit of a transferase that will attach a lipid from a lipid donor to a protein;
- modifying the host organism to include a second gene expressing the protein, wherein the protein includes a substrate of the transferase;
- providing the lipid donor;
- and
- culturing the host organism to express the first gene and the second gene in the presence of the lipid donor to form the lipidated protein.
Dependencies are inferred from the claim text.