US 2025/0066828 A1Published application

C-nucleoside monophosphate synthesis

AssigneesUAB Biomatter Designs, Enginzyme AB

InventorsMartyna KOPLUNAITE, Nina URBELIENE, Rolandas MEŠKYS, Diana IKASALAITE, Linas ZAKRYS, Laurynas KARPUS, Federica RUGGIERI, Sascha GROBE

Life of this patent

Estimating the term…

patentgrep

Loading patent…

Abstract

Provided herein is a one-pot method of synthesising a C-nucleoside-5′-monophosphate such as pseudouridine-5′-monophosphate, from an N-nucleoside using a multi-step enzymatic pathway. The method comprises reacting the N-nucleoside with a phosphate source and nucleoside phosphorylase to form a pentose-1-phosphate intermediate, reacting the pentose-1-phosphate intermediate with a phosphomutase to form pentose-5-phosphate intermediate, and reacting the pentose-5-phosphate intermediate with a nucleoside-5′-phosphate C-glycosidase to form the C-nucleoside-5′-monophosphate.

Claims38

Dependent claims

A method of synthesising a C-nucleoside-5′-monophosphate, the method comprising:

a) reacting an N-nucleoside with a source of phosphate in the presence of a nucleoside phosphorylase to form a first intermediate, wherein the nucleoside comprises a pentose sugar and a first base, and wherein the first intermediate comprises a pentose-1-phosphate;
b) reacting the first intermediate comprising a pentose-1-phosphate with a phosphomutase to form a second intermediate, wherein the second intermediate comprises a pentose-5-phosphate;
and
c) reacting the second intermediate comprising a pentose-5-phosphate with a second base in the presence of a nucleoside-5′-monophosphate C-glycosidase to form the C-nucleoside-5′-monophosphate.

1 more element

Dependencies are inferred from the claim text.