Cyp72a219 genes conferring herbicide tolerance
AssigneesColorado State University Research Foundation, Monsanto Technology LLC, The Board of Trustees of the University of Illinois
InventorsTodd GAINES, Carlos RIGON, Franck DAYAN, Anita Küpper, Roland BEFFA, Darci GIACOMINI, Patrick TRANEL
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Abstract
The present disclosure relates to a plant or plant part comprising increased expression of a polynucleotide encoding a CYP72A219 polypeptide that confers to the plant or plant part tolerance to HPPD inhibitor herbicides, such as tembotrione or mesotrione. The disclosure further provides kits for identifying herbicide resistant plants and methods for determining whether a plant is herbicide resistant.
Claims72
A modified plant, or a progeny, plant seed, plant part, or plant cell thereof, having tolerance to an herbicide, the modified plant having increased expression of a polynucleotide encoding a cytochrome P450 72A219 (CYP72A219) polypeptide relative to an unmodified plant, progeny, plant seed, plant part, or plant cell of the same species.
A nucleic acid molecule comprising:
- (a) a nucleotide sequence encoding a CYP72A219 polypeptide, wherein the nucleotide sequence has at least 80%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO:
- 1 or 3;
- or
- (b) a nucleotide sequence encoding a CYP72A219 polypeptide, wherein the CYP72A219 polypeptide has at least 80%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO:
- 2 or 4.
A CYP72A219 polypeptide comprising an amino acid sequence having at least 80%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO:
- 2 or 4.
A method for producing a plant with herbicide tolerance, the method comprising:
- increasing expression of a polynucleotide encoding a CYP72A219 polypeptide in the plant, wherein the herbicide tolerance of the plant is increased when compared to a plant that lacks the increased expression.
A method for controlling undesired vegetation at a plant cultivation site, the method comprising:
- providing at the site a plant that comprises a polynucleotide encoding a CYP72A219 polypeptide, wherein expression of the polynucleotide confers to the plant tolerance to an herbicide;
- and
- applying to the site an effective amount of the herbicide.
A method for controlling the growth of an herbicide resistant weed at a plant cultivation site, the method comprising:
- contacting the weed with a composition that reduces expression or activity of a CYP72A219 polypeptide;
- and
- applying to the site an effective amount of the herbicide.
A method for identifying an herbicide-resistant plant, the method comprising:
- obtaining a sample from a plant suspected of having herbicide resistance;
- quantifying expression of a CYP72A219 gene in the sample, wherein the CYP72A219 gene is differentially expressed in an herbicide-resistant plant compared to an herbicide-sensitive plant of the same species;
- and
- determining that the plant is herbicide-resistant based on the quantification.
A method for identifying an herbicide-resistant plant, the method comprising:
- obtaining a sample from a plant suspected of having herbicide resistance;
- detecting one or more motifs in the promoter of a CYP72A219 gene in the sample, wherein the motif comprises the nucleotide sequence of SEQ ID NO:
- 34, 35, 36, 37, 38, 39, 40, or 41;
- and
- determining that the plant is herbicide-resistant based on the presence of the one or more motifs.
A kit for identifying an herbicide-resistant plant, the kit comprising at least two primers, wherein the at least two primers recognize a CYP72A219 gene that is differentially expressed in an herbicide-resistant plant compared to an herbicide-sensitive plant of the same species.
Dependencies are inferred from the claim text.