Functional DNA Cassette and Plasmid

Abstract

The present invention mainly relates to a DNA cassette comprising: a replication origin sequence capable of binding to an enzyme with DnaA activity; and a first promoter sequence; wherein transcription from the first promoter sequence flows into the replication origin sequence, and the distance between the 3′ terminal base of the first promoter sequence and the terminal base of the replication origin sequence is within 450 bases. The invention also relates to a plasmid comprising: a replication origin sequence capable of binding to an enzyme with DnaA activity; a first promoter sequence; and a plasmid replication origin; wherein transcription from the first promoter sequence flows into the replication origin sequence, and the distance between the 3′ terminal base of the first promoter sequence and the terminal base of the replication origin sequence is within 2000 bases.

Dependencies are inferred from the claim text.