Systems and Methods Incorporating Modified T-Cells
OwnerMark M. Davis, Xin Chen +2inventors
InventorsMark M. Davis, Xin Chen, Mustafa Ghanizada, Venkata Vamsee Aditya Mallajosyula
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Abstract
Disclosed herein are methods, systems and devices to model adaptive immune responses and develop and/or test improved antibodies, vaccines, and other therapeutic agents. The adaptive immune responses can be modeled using lymphoid tissue derived from a subject. The methods, systems and devices disclosed herein can comprise modified T-cells.
Claims51
An in vitro cell cluster comprising lymphoid cells,
- wherein said in vitro cell cluster derived from lymphoid tissue of a subject, said in vitro cell cluster comprising a germinal center configured to produce antibodies to an antigen, wherein said germinal center comprises genetically modified T-cells.
A method for generating antibodies from a lymphoid organoid, comprising:
- (a) placing lymphoid cells in a media to produce said lymphoid organoid, wherein said lymphoid organoid comprises T-cells and B-cells, wherein said T-cells are modified to have a decreased regulation of B-cells as compared to unmodified T-cells;
- (b) introducing an antigen to said media;
- (c) incubating said lymphoid organoid with said antigen to generate said antibodies;
- and
- (d) isolating said antibodies or nucleic acids encoding said antibodies from said lymphoid organoid.
An in vitro cell cluster comprising lymphoid cells, wherein said in vitro cell cluster is derived from lymphoid tissue of a subject, said in vitro cell cluster comprising a germinal center configured to produce antibodies to a self-antigen, wherein said germinal center comprises genetically modified T-cells.
A method for generating antibodies from a lymphoid organoid, comprising:
- (a) placing lymphoid cells in a media to produce said lymphoid organoid, wherein said lymphoid organoid comprises T-cells and B-cells, wherein said T-cells are modified to have a decreased regulation of B-cells as compared to unmodified T-cells;
- (b) introducing a self-antigen to said media;
- (c) incubating said lymphoid organoid with said self-antigen to generate said antibodies;
- and
- (d) isolating said antibodies from said lymphoid organoid.
Dependencies are inferred from the claim text.