Composition for separating extracellular vesicles comprising amine-functionalized solid support and homobifunctional hydrazide

Abstract

Proposed are an extracellular vehicle-isolating composition including a homobifunctional hydrazide and a solid support functionalized with amine groups, and a method of isolating extracellular vesicles by using the composition. The method may isolate highly enriched high-purity EVs from a biological sample and also enable simultaneous operation for EVs isolation and extraction of EV-derived proteins or EV-derived nucleic acids. The method is low-cost and simple, does not require special equipment other than a syringe filter, and allows for high-throughput experiments due to the use of large-volume samples. The method may reduce the time required for protein and nucleic acid extraction by simplifying the experiments. Additionally, this EV enrichment may increase the sensitivity of biomarker detection for disease diagnosis and treatment.

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