Imaging method for designer receptor, antagonist or agonist, pharmaceutical, and companion diagnostic agent

A method for imaging axon terminals of a nerve cell having an introduced gene encoding a mutated human M4 muscarinic acetylcholine receptor (hM4D) or a mutated human M3 muscarinic acetylcholine receptor (hM3D) in a brain of a live animal subject, the method comprising:

• allowing the compound represented by Formula (II) or a pharmaceutically acceptable salt or solvate thereof to migrate into the brain to selectively bind to the hM4D or the hM3D

An antagonist or an agonist comprising:

• a substance that selectively binds to a mutated human M4 muscarinic acetylcholine receptor (hM4D) or a mutated human M3 muscarinic acetylcholine receptor (hM3D) introduced into a cell in a brain of a live animal subject,
• the substance being a compound represented by Formula (I) or a pharmaceutically acceptable salt or solvate thereof:
• wherein one or more atoms are or are not radioisotopes of the atom or atoms.

A pharmaceutical comprising:

• a substance that selectively binds to a mutated human M4 muscarinic acetylcholine receptor (hM4D) or a mutated human M3 muscarinic acetylcholine receptor (hM3D) introduced into a cell in a brain of a live primate subject,
• the substance being a compound represented by Formula (I) or a pharmaceutically acceptable salt or solvate thereof:
• wherein one or more atoms are or are not radioisotopes of the atom or atoms.

A companion diagnostic agent comprising:

• a substance that selectively binds to a mutated human M4 muscarinic acetylcholine receptor (hM4D) or a mutated human M3 muscarinic acetylcholine receptor (hM3D) introduced into a cell in a brain of a live primate subject,
• the substance being a compound represented by Formula (I) or a pharmaceutically acceptable salt or solvate thereof:
• wherein one or more atoms are or are not radioisotopes of the atom or atoms.