Synergistic bacterial compositions and methods of production and use thereof
AssigneeSeres Therapeutics, Inc.
InventorsMatthew R. HENN, Geoffrey von MALTZAHN, Anthony Mario D'ONOFRIO, Kevin Daniel LITCOFSKY, David Arthur BERRY, David N. COOK, Noubar B. AFEYAN, John Grant AUNINS
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Abstract
Provided are therapeutic compositions containing Ecobiotic™ populations for prevention, treatment and reduction of symptoms associated with a dysbiosis of a mammalian subject such as a human.
Claims20
A composition comprising a first species of isolated bacterium and a second species of isolated bacterium, wherein the first species and the second species are not identical, and wherein the first species and the second species independently comprise a 16S rDNA sequence that is at least 97% identical to a 16S rDNA sequence present in a reference bacterium selected from Table 3 with the proviso that:
- (i) if the first species is Clostridium innocuum, then the second species is not Clostridium orbiscindens,
- (ii) if the first species is Clostridium orbiscindens, then the second species is not Clostridium innocuum, Lachnospiraceae_bacterium_5_1_57FAA, Dorea longicatena, nor Clostridium symbiosum,
- (iii) if the first species is Lachnospiraceae_bacterium_5_1_57FAA, then the second species is not Clostridium orbiscindens nor Clostridium disporicum,
- (iv) if the first species is Dorea longicatena, then the second species is not Clostridium orbiscindens,
- (v) if the first species is Clostridium disporicum, then the second species is not Lachnospiraceae_bacterium_5_1_57FAA,
- or
- (vi) if the first species is Clostridium symbiosum, then the second species is not Clostridium orbiscindens.
(canceled)
A single dose unit comprising a first species of isolated bacterium and a second species of isolated bacterium, wherein the first species and the second species are capable of inhibiting Clostridium difficile (C. difficile) growth as measured by a CivSim assay and/or populating the gastrointestinal tract of a subject, where in the first species and the second species are not identical, and wherein the first species and the second species independently comprise a 16S rDNA sequence that is at least 97% identical to a 16S rDNA sequence present in a reference bacterium selected from Table 3 with the provisio that:
- (i) if the first species is Clostridium innocuum, then the second species is not Clostridium orbiscindens,
- (ii) if the first species is Clostridium orbiscindens, then the second species is not Clostridium innocuum, Lachnospiraceae_bacterium_5_1_57FAA, Dorea longicatena, nor Clostridium symbiosum,
- (iii) if the first species is Lachnospiraceae_bacterium_5_1_57FAA, then the second species is not Clostridium orbiscindens nor Clostridium disporicum,
- (iv) if the first species is Dorea longicatena, then the second species is not Clostridium orbiscindens,
- (v) if the first species is Clostridium disporicum, then the second species is not Lachnospiraceae_bacterium_5_1_57FAA,
- or
- (vi) if the first species is Clostridium symbiosum, then the second species is not Clostridium orbiscindens.
A method of producing a bacterial composition, comprising combining a first species of isolated bacterium and a second species of isolated bacterium, wherein the first species and the second species are not identical, wherein the first species and the second species are capable of inhibiting Clostridium difficile (C. difficile) growth and/or populating the gastrointestinal tract of a subject, and wherein the first species and the second species independently comprise a 16S rDNA sequence that is at least 97% identical to a 16S rDNA sequence present in a reference bacterium selected from Table 3 with the proviso that:
- (i) if the first species is Clostridium innocuum, then the second species is not Clostridium orbiscindens,
- (ii) if the first species is Clostridium orbiscindens, then the second species is not Clostridium innocuum, Lachnospiraceae_bacterium_5_1_57FAA, Dorea longicatena, nor Clostridium symbiosum,
- (iii) if the first species is Lachnospiraceae_bacterium_5_1_57FAA, then the second species is not Clostridium orbiscindens nor Clostridium disporicum,
- (iv) if the first species is Dorea longicatena, then the second species is not Clostridium orbiscindens,
- (v) if the first species is Clostridium disporicum, then the second species is not Lachnospiraceae_bacterium_5_1_57FAA,
- or
- (vi) if the first species is Clostridium symbiosum, then the second species is not Clostridium orbiscindens.
Dependencies are inferred from the claim text.