Method for isolating and purifying neophytadiene from leaf tobacco, neophytadiene composition, non-combustion heating-type flavor inhaler, non-combustion non-heating type flavor inhaler, combustion-type flavor inhaler and methods for producing same

A method of performing isolated purification of neophytadiene from leaf tobacco, the method comprising:

• a step of obtaining an extract (A1) by adding an aprotic solvent to the leaf tobacco to perform extraction operation and then removing an extraction residue;
• a step of obtaining an extract (B1) by adding an acidic aqueous solution to the extract (A1) to perform liquid-liquid extraction and then removing an aqueous layer;
• a step of obtaining an extract (C1) by adding a protic solvent to the extract (B1) to perform liquid-liquid extraction and then removing a layer of a protic solvent;
• a step of obtaining a dry solid (D1) by removing a solvent of the extract (C1);
• a step of obtaining a solution (E1) by adding a protic solvent to the dry solid (D1) to dissolve the dry solid (D1);
• a step of obtaining a supernatant (F1) by removing a deposit produced in the solution (E1);
• a step of obtaining a solution (G1) by replacing a solvent of the supernatant (F1) with an aprotic solvent;
• and
• a step of performing isolated purification of neophytadiene from the solution (G1) through normal phase high performance liquid chromatography.