Self-Fertilization as Methods for Stabilizing Ploidy after Colchicine Treatment Model

Abstract

Exemplary methods include a method of stabilizing ploidy including doubling a plant's ploidy, self-fertilizing the doubled plant, and creating an offspring with a germline ploidy of a desired doubled ploidy targeted when doubling the plant's ploidy. Another exemplary method of stabilizing ploidy includes self-fertilizing a haploid plant, and creating an offspring plant with a germline ploidy that is diploid. A further method of stabilizing ploidy includes treating a female microtubule inhibitor treated plant with an ethylene blocker, producing male reproductive organs and pollen by the female microtubule inhibitor treated plant, fertilizing a clone of the female microtubule inhibitor treated plant with the pollen, and producing seeds and/or offspring.

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