A method for providing source data for training or validating a processing model for processing analyte image sequences, wherein an analyte image sequence is generated by labeling analytes with markers in a plurality of coloring rounds and detecting the markers with a camera, the markers are selected based on a codebook such that signal sequences of analytes in an image region over the analyte image sequence comprise colored signals and uncolored signals, the camera captures an image of the analyte image sequence in each coloring round, the method comprising:
- capturing a spot analyte image sequence of a sample,
- providing an evaluation of image regions based on image signals of the image regions,
- identifying one or more spot image regions in the spot images from the evaluation of the image regions, wherein the evaluation of spot image regions of spot images for colored signals has a spot evaluation;
- capturing one or more source analyte image sequences comprising source images registered to the spot images, comprising reducing a scene contrast in order to record the source images registered to the spot images with a scene contrast reduced compared to the spot images, such that the evaluation of the spot image regions based on the image signals of the spot image regions for the source images of at least one of the source analyte image sequences for colored signals has a non-spot evaluation, and
- providing the at least one of the source analyte image sequences as source data.