Methods of Culturing and Characterizing Antibody Secreting Cells
AssigneeEmory University
InventorsFrances Eun-Hyung Lee, Ignacio Sanz, Doan C. Nguyen
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Abstract
This disclosure relates to methods of culturing and characterizing antibody secreting cells. In certain embodiments, this disclosure relates to methods of isolating antibody secreting cells, e.g., long lived plasma cells, replicating the isolated cells in growth media disclosed herein, and determining the nucleic acids sequences in the cells that encode the produced antibodies.
Claims9
A method of determining the effectiveness of a vaccination comprising administering a vaccine to a subject that results in the existence or production of a vaccine antigen in the subject;
- isolating antibody secreting cells from a sample, providing isolated antibody secreting cells;
- mixing the isolated antibody secreting cells with secretions of allogeneic mesenchymal stromal/stem cells and exogenously added A-proliferation-inducing ligand (APRIL) under conditions such that isolated antibody secreting cells replicate providing replicated antibody secreting cells;
- identifying replicated antibody secreting cells that produce antibodies that bind to the vaccine antigen;
- wherein time between administering the vaccine and isolating the antibody secreting cells from the sample is at least one year or more;
- and
- determining the vaccination is effective if the antibody secreting cells have a cell surface profile of CD19(−), CD38(hi), and CD138(+).
A method of determining the efficacy of an autoimmune therapy comprising:
- administering an autoimmune drug to a subject diagnosed with an autoimmune disease;
- isolating antibody secreting cells from a sample of the subject, providing isolated antibody secreting cells;
- mixing the isolated antibody secreting cells with secretions of allogeneic mesenchymal stromal/stem cells and exogenously added A-proliferation-inducing ligand (APRIL) under conditions such that isolated antibody secreting cells replicate providing replicated antibody secreting cells;
- identifying replicated antibody secreting cells that produce auto-antibodies that specifically bind to an autoimmune antigen associated with the diagnosed autoimmune disease;
- and
- correlating the amount of auto-antibodies or cells that produce auto-antibodies in the sample to the efficacy of the autoimmune therapy.
Dependencies are inferred from the claim text.