Method for detecting structural polymorphism mutations

Abstract

Provided is a method and a means for improving multiplex simultaneous detection of a structural polymorphism mutation in capillary electrophoresis. In one embodiment, the present invention relates to a method for detecting a mutation in a test nucleic acid, the method including: providing a first primer set, containing at least one labeled first primer, for amplifying a nucleic acid with a first mutation; providing a second primer set, containing at least one labeled second primer, for amplifying a nucleic acid with a second mutation; amplifying the test nucleic acid with use of the first primer set and the second primer set; and subjecting an obtained amplification product to capillary electrophoresis to analyze the mutation in the test nucleic acid, the first primer and the second primer being individually labeled with combinations of different numbers and/or kinds of fluorescent dyes.

Dependencies are inferred from the claim text.