Flow cytometer, discrimination method, and program

A discrimination method of discriminating a measurement object based on a result detected by a flow cytometer including:

• a flow channel allowing a measurement object not labeled with a fluorescent dye to flow along with a fluid;
• a light source emitting illumination light;
• an illumination optical system irradiating the measurement object flowing in the flow channel with the illumination light emitted from the light source as a spotlight which is illumination light condensed in at least one of a width direction and a depth direction of the flow channel at an irradiation position in the flow channel;
• a light detector detecting fluorescence emitted from a specific molecule of the measurement object in response to irradiation with the spotlight via the illumination optical system;
• and
• a detection optical system allowing the fluorescence to propagate to the light detector, the discrimination method comprising:
• an acquisition step of acquiring data indicating an intensity of the fluorescence detected by the light detector for the measurement object flowing in the flow channel;
• a generation step of generating information of the fluorescence based on the data acquired in the acquisition step;
• and
• a discrimination step of discriminating whether the measurement object is a target measurement object based on the information of the fluorescence generated in the generation step.

A program causing a computer to perform information processing based on a result detected by a flow cytometer including:

• a flow channel allowing a measurement object not labeled with a fluorescent dye to flow along with a fluid;
• a light source emitting illumination light;
• an illumination optical system irradiating the measurement object flowing in the flow channel with the illumination light emitted from the light source as a spotlight which is illumination light condensed in at least one of a width direction and a depth direction of the flow channel at an irradiation position in the flow channel;
• a light detector detecting fluorescence emitted from a specific molecule of the measurement object in response to irradiation with the spotlight via the illumination optical system;
• and
• a detection optical system allowing the fluorescence to propagate to the light detector, the computer performing:
• an acquisition step of acquiring data indicating an intensity of the fluorescence detected by the light detector for the measurement object flowing in the flow channel;
• a generation step of generating information of the fluorescence based on the data acquired in the acquisition step;
• and
• a discrimination step of discriminating whether the measurement object is a target measurement object based on the information of the fluorescence.